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D.R. Taylor, N. Gupta, Y.H. Yucel; Acute and Transient Elevated Intraocular Pressure Induces Oxidative Damage in the Rat Superior Colliculus . Invest. Ophthalmol. Vis. Sci. 2003;44(13):140.
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Purpose: To determine whether acute and transient raised pressure within the eye induces oxidative damage in the brain, specifically peroxynitrite-mediated injury in the rat superior colliculus. Methods: In the right eye of adult rats (n = 8), intraocular pressure was acutely raised to 110 mmHg for 50 minutes. The survival times of animals following this insult ranged from 2.6 months to 1.3 years, and optic nerves and brains were perfusion fixed under the same conditions. The midbrain was serially sectioned and right and left superior colliculi sections from each animal were stained for nitrotyrosine using immunohistochemistry. The peroxynitrite-mediated damage was quantitatively assessed with counts of nitrotyrosine immunoreactive profiles. Numbers of profiles in the left superior colliculus connected to the right injured eye were compared to those counted in the left superior colliculus connected mainly to the unaffected fellow eye using the non-parametric Wilcoxon rank sum test. Results: The number of nitrotyrosine immunoreactive profiles in the left superior colliculus was greater than that seen in the right superior colliculus, for each of the animals with various survival times following the injury (89.7 ± 7.6 vs. 43.8 ± 4.4;mean ± SEM). The average ratio of left/right superior colliculus nitrotyrosine immunoreactive profiles was significantly increased (2.15 ± 0.22; p = 0.004). Conclusion: Acute and transient elevated intraocular pressure in the rat at a sufficient level to cause retinal ischemia, induces oxidative damage in the major vision center of the brain. Supported in part by Glaucoma Research Foundation (San Francisco), Glaucoma Research Society of Canada, Toronto Lions Club, Anewman Memorial Foundation, St. Michael's Hospital Volunteer Association
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