May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
The Neuroprotective Effect of Ginkgo Biloba Extract on Retinal Ganglion Cells in a Rat Glaucoma Model
Author Affiliations & Notes
  • V.W. Lee
    Ophthalmology, Hong Kong Adventist Hospital, Hong Kong, Hong Kong Special Administrative Region of China
  • C. Lai
    Anatomy, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region of China
  • H.K. Yip
    Anatomy, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region of China
  • W. Wu
    Anatomy, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region of China
  • K.F. So
    Anatomy, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative Region of China
  • Footnotes
    Commercial Relationships  V.W. Lee, None; C. Lai, None; H.K. Yip, None; W. Wu, None; K.F. So, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 146. doi:
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      V.W. Lee, C. Lai, H.K. Yip, W. Wu, K.F. So; The Neuroprotective Effect of Ginkgo Biloba Extract on Retinal Ganglion Cells in a Rat Glaucoma Model . Invest. Ophthalmol. Vis. Sci. 2003;44(13):146.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigate the neuroprotective effect of Ginkgo biloba extract (GBE) on retinal ganglion cells (RGCs) in a rat glaucoma model with increased intraocular pressure (IOP) induced by laser photocoagulation of the aqueous veins. Methods: Elevated IOP observed in the right eye of SD rats was induced by laser photocoagulation of the episcleral and limbal veins. The laser treatments were conducted twice with 7 days separation. After the first laser treatment, the animals were then received daily oral administration of: (1) vehicle (0.01M PBS); (2) 1.2mg/ml GBE; (3) 12mg/ml GBE; (4) 120mg/ml GBE; (5) 200mg/ml GBE for 14 days. Standardized batches of GBE were purchased from CV Technologies, Canada. Three days before the sacrifice of animals, a piece of gelfoam soaked with 6% Fluoro-Gold was applied to the superior colliculi of those animals. After a survival of 2 weeks, the animals were killed and the retinas were flat-mounted. Results: In all five groups, the number of RGCs was counted in three quadrants (nasal, inferior & temporal) of all the retinas and the changes in the densities of RGCs were expressed as percentage loss of RGCs by comparing the laser-treated eyes with the contralateral, control eyes of the same animal. There was no loss of RGC in the group with 12, 120 and 200mg/ml GBE but there was a loss of 13% in the vehicle group. There was a significant difference between 12 (p<0.001); 120 (p<0.001); 200 (p<0.001) mg/ml GBE and PBS groups, but there was no significant GBE administered orally may provide neuroprotection against glaucoma induced retinal damage in rats.difference between 1.2mg/ml GBE and PBS groups (p>0.05). Conclusions: GBE administered orally may provide neuroprotection against glaucoma induced retinal damage in rats.

Keywords: neuroprotection • intraocular pressure • ganglion cells 
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