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Z. Yang, K. Yu, Z. Wang, J. Zhuang, L. Yu; Retinal Cells of Rat Neonates Indu . Invest. Ophthalmol. Vis. Sci. 2003;44(13):150.
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© ARVO (1962-2015); The Authors (2016-present)
Objective: To investigate the inducing action of retinal cells of rat neonates on the adult rat bone marrow stromal cells(BMSCs ) Methods: The full marrow from adult Wistar rat were cultured and passaged repeatedly to harvest the pure BMSCs. In vitro, the BMSCs were induced by retinal cells of rat neonates in transwell . The morphological changes of the BMSCs were observed under phase contrast microscope, the specific markers of the induced cells were identified immunocytochemically with Nestin,Map-2, neuron-specific enolase (NSE), neurofilament(NF)Thy1.1 and glial fibrillary acidic protein(GFAP) antibodies. And the mRNA of Nestin, NF, NSE, Thy1.1 and Ran were detected in the induced cells by RT-PCR. Results: The BMSCs can be induced by reinal cells of rat neonates into the retina-like neurons which have the typical neural morphology and expression of the specific retinal neural antigens. Conclusions: The BMSCs from adult rat can be induced into retina-like cells by retinal cells of rat neonates .
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