May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Retinal Cells of Rat Neonates Indu
Author Affiliations & Notes
  • Z. Yang
    Zhongshan Ophthalmic Center, Sun Yat-Sen Univ Medical Sci, Guangzhou, China
  • K. Yu
    Zhongshan Ophthalmic Center, Sun Yat-Sen Univ Medical Sci, Guangzhou, China
  • Z. Wang
    Zhongshan Ophthalmic Center, Sun Yat-Sen Univ Medical Sci, Guangzhou, China
  • J. Zhuang
    Department of biochemistry,Zhongshan Medical college, Sun Yat-Sen Univ Medical Sci, Guangzhou, China
  • L. Yu
    Department of biochemistry,Zhongshan Medical college, Sun Yat-Sen Univ Medical Sci, Guangzhou, China
  • Footnotes
    Commercial Relationships  Z. Yang, None; K. Yu, None; Z. Wang, None; J. Zhuang, None; L. Yu, None.
  • Footnotes
    Support  Chinese National Grant of Science
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 150. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Z. Yang, K. Yu, Z. Wang, J. Zhuang, L. Yu; Retinal Cells of Rat Neonates Indu . Invest. Ophthalmol. Vis. Sci. 2003;44(13):150.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Objective: To investigate the inducing action of retinal cells of rat neonates on the adult rat bone marrow stromal cells(BMSCs ) Methods: The full marrow from adult Wistar rat were cultured and passaged repeatedly to harvest the pure BMSCs. In vitro, the BMSCs were induced by retinal cells of rat neonates in transwell . The morphological changes of the BMSCs were observed under phase contrast microscope, the specific markers of the induced cells were identified immunocytochemically with Nestin,Map-2, neuron-specific enolase (NSE), neurofilament(NF)Thy1.1 and glial fibrillary acidic protein(GFAP) antibodies. And the mRNA of Nestin, NF, NSE, Thy1.1 and Ran were detected in the induced cells by RT-PCR. Results: The BMSCs can be induced by reinal cells of rat neonates into the retina-like neurons which have the typical neural morphology and expression of the specific retinal neural antigens. Conclusions: The BMSCs from adult rat can be induced into retina-like cells by retinal cells of rat neonates .

Keywords: neuroprotection • retinal culture 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×