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K. Amemiya, M. Takahashi, M. Haruta, Y. Honda; Young Adult Human Retinal Pigment Epithelial Cells Have Ability of Transdifferentiation into Neuron In Vitro . Invest. Ophthalmol. Vis. Sci. 2003;44(13):499.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: It is reported that the retinal pigmented epithelial cells (RPE cells) of many vertebrate species transdifferentiate into lens cells in vitro and that embryonic rat RPE cells can differentiate into retinal neurons in vitro. The purpose of this study is to investigate whether the adult human RPE cells have the ability to transdifferentiate into neurons and glias. Methods:We used a clonal RPE cell line (ARPE-19) that was isolated from 19 year-old young adult eye. After we passaged the cells several times in the medium with fetal bovine serum, the medium was replaced with various components to provide conditions that induce neuronal differentiation. To investigate the property of the cells before and after transdifferentiation, we employed immunocytochemical analysis and used antibodies to beta III tubulin, microtubule associated protein 5 (MAP5), neurofilament 200 (NF200), glial fibrillary acidic protein (GFAP) and rhodopsin. Results:The ARPE-19 cells maintained the epithelial shape and stably proliferated in control medium with fetal bovine serum. Their shape changed into elongated one after a several days in serum free medium with basic fibroblast growth factor and epidermal growth factor. They formed multiple processes with retinoic acid treatment. The most of the cells were beta III tubulin-positive even before differentiation. We observed not only beta III tubulin-positive but also MAP5- and NF200-positive cells after differentiation. However, no GFAP- nor rhodopsin-positive cells appeared. Conclusions:Cultured adult human RPE cells were beta III tubulin-positive. They possess a potency to differentiate into neurons but not into glias with this condition.
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