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C. Yang, C. Yang; Inhibition of Bovine Retinal Capillary Endothelial Cell Mediated Angiogenesis by Disintegrin, a vß3 Integrin Antagonist from Snake Venom . Invest. Ophthalmol. Vis. Sci. 2003;44(13):555.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Retinal angiogenesis occurs in a variety of disease, such as proliferative diabetic retinopathy, retinopathy of prematurity, and uveitis. A αvß3 integrin dependent vascular endothelial cell migration and proliferation may play an important role in the pathogenesis of new vessel formation. Disintegrin, a group of RGD-containing peptides from viper venom, is a strong antagonist of αvß3 integrin. In this study, the inhibitory effect of disintegrin on angiogenesis was evaluated in vitro by a bovine retinal capillary endothelial (BRCE) cell culture model. Methods: BRCE cell culture was established in the experiment. The inhibitory effect of disintegrin on BRCE cell mediated angiogenesis was evaluated by the cell adhesion assay, chemotatic cell migration assay and capillary-like tube formation in Matrix-gel assay. Results: Disintegrin, echstatin and flavoridin, showed dose-dependently inhibitory effect on BRCE cell mediated angiogenesis in the cell adhesion assay, chemotatic cell migration assay and capillary-like tube formation in Matrix-gel assay. The potency of disintegrin was 100 to 500 times higher than that of GRGDS peptide and momoclonal anti-αvß3 antibody LM609. The inhibitory effect of disintegrin on BRCE cell was through the apoptosis pathway. Conclusions: Disintegrin, a αvß3 integrin antagonist, effectively inhibit BRCE cell mediated angiogenesis. Disintegrins and their analogs may be a potential therapeutic agent for the treatment of retinal angiogenesis disorders.
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