May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
A Novel Bovine Eye Angiogenesis Assay
Author Affiliations & Notes
  • M.D. Conway
    Ophthalmology, Tulane University Health Sciences Center, New Orleans, LA, United States
  • J. Lewis
    Surgery, Louisiana State University Health Sciences Center, New Orleans, LA, United States
  • G.A. Peyman
    Surgery, Louisiana State University Health Sciences Center, New Orleans, LA, United States
  • Y. Bezerra
    Surgery, Louisiana State University Health Sciences Center, New Orleans, LA, United States
  • C. Anthony
    Surgery, Louisiana State University Health Sciences Center, New Orleans, LA, United States
  • E. Woltering
    Surgery, Louisiana State University Health Sciences Center, New Orleans, LA, United States
  • Footnotes
    Commercial Relationships  M.D. Conway, None; J. Lewis, Louisiana State University Health Sciences Center P; G.A. Peyman, None; Y. Bezerra, None; C. Anthony, Louisiana State University Health Sciences Center P; E. Woltering, Louisiana State University Health Sciences Center P.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 563. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M.D. Conway, J. Lewis, G.A. Peyman, Y. Bezerra, C. Anthony, E. Woltering; A Novel Bovine Eye Angiogenesis Assay . Invest. Ophthalmol. Vis. Sci. 2003;44(13):563.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: In most situations, angiogenesis in humans is a pathologic state, including tumor growth, a variety of skin disorders, and of particular interest, age-related macular degeneration and diabetic retinopathy. Whereas various in vitro models have evaluated angiogenesis, few have evaluated the angiogenic potential of specific anatomical portions of the eye. We hypothesized that portions of a bovine eye, when embedded in a 0.3% fibrin thrombin clot and supplemented with Media 199 and fetal bovine serum, would demonstrate angiogenesis. This assay has previously proven to be effective with other tissues such as human placental vein and various tumors. Methods: Eight fresh bovine eyes were obtained from a slaughterhouse and transported to the laboratory on ice. The eyes were dissected to evaluate various components of the bovine eye for angiogenic potential, including sclera, choroid/retinal pigment epithelium (RPE)/sclera, choroid/RPE, choroid/sclera, and retina. After being embedded in a fibrin thrombin clot in 96 well plates and incubated with Media 199 with serum for 15 days, the tissues were evaluated for the initiation of angiogenesis and subsequent proliferation of angiogenesis. Results: Angiogenesis initiated on day 6 in the choroid/RPE-containing wells, which was more rapid than in human tumor or other normal tissue models (human umbilical vein and human placenta). Interestingly, retina alone did not initiate angiogenesis in any wells. The angiogenesis was greatest for wells containing RPE/choroid. Conclusions: We have developed a novel bovine retinal and choroidal angiogenesis assay that may be useful to evaluate agents for the treatment of retinal neovascularization. The response of these organ-specific neovessels to antiangiogenics may be more predictive than responses seen in other non-organ-specific assay systems. Initiation of Angiogenesis by Various Ocular Tissues  

Keywords: retinal neovascularization • age-related macular degeneration • diabetic retinopathy 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×