May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
ROS-GC1/GCAP1 Signal Transduction Machinery in the Photoreceptor-Bipolar Synaptic Region
Author Affiliations & Notes
  • R. Sharma
    Dept of Cell Biology, Univ of Med & Dentistry of NJ, Stratford, NJ, United States
  • V. Venkataraman
    Dept of Cell Biology, Univ of Med & Dentistry of NJ, Stratford, NJ, United States
  • T. Duda
    Dept of Cell Biology, Univ of Med & Dentistry of NJ, Stratford, NJ, United States
  • N. Vardi
    Dept of Neuroscience, University of Pennsylvania, Philadelphia, PA, United States
  • K. Koch
    Fordchungszentrum Juelich, Institut for Biologische Informationsverarbeitung, Juelich, Germany
  • Footnotes
    Commercial Relationships  R. Sharma, None; V. Venkataraman, None; T. Duda, None; N. Vardi, None; K. Koch, None.
  • Footnotes
    Support  NIH grants EY10828 and DC005349; DFG grant Ko948/5-3
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1068. doi:
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      R. Sharma, V. Venkataraman, T. Duda, N. Vardi, K. Koch; ROS-GC1/GCAP1 Signal Transduction Machinery in the Photoreceptor-Bipolar Synaptic Region . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1068.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:Rod outer segment membrane guanylate cyclase (ROS-GC) transduction system is composed of two parts, Ca2+ sensor guanylate cyclase activating protein (GCAP), and ROS-GC. The system is pivotal for the Ca2+-sensitive phototransduction machinery. This study was designed to examine if this system is also operational in the photoreceptor-bipolar synaptic region. Methods:A combination of biochemical analyses involving enzyme assays, reconstitution, peptide competition, cross-linking and immunocytochemical analyses has been used. Results:The results show that the GCAP1/ROS-GC1 transduction system exists in the photoreceptor synaptic (presynaptic) terminals. Thus, the presence of this system and its linkage is not unique to the phototransduction machinery. A new topographic model of ROS-GC1 transduction is proposed. In this model, the catalytic module of ROS-GC1 at its opposite ends is independently regulated by GCAP1- and S100ß-modules. GCAP1 senses the Ca2+ impulse and inhibits the catalytic module; S100ß senses the impulse and stimulates the catalytic module. Conclusions: ROS-GC1 acts as a bimodal Ca2+ signal transduction switch in the photoreceptor bipolar synapse.

Keywords: photoreceptors • calcium • signal transduction 
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