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X. Zhao, P. Russell; Effects of Prostaglandin Analogues on the FP Receptor and Genes Associated with Glaucoma . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1149.
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Purpose: To determine the effects of prostaglandin analogues on the FP receptor and genes associated with glaucoma in human ciliary muscle (HCM) and trabecular meshwork (HTM) cells. Methods: Cultures of HCM and HTM cells were established from five different donors, treated for nine days with 10 µg/ml of either latanoprost free acid or prostaglandin F2α ethanolamide and compared to control cells. The mRNA from the cells of the five individual donors was extracted and purified. Gene expression changes were performed by either Real-Time PCR or relative quantitative PCR. Results: The mRNA levels for the prostaglandin FP receptor were down regulated in the human ciliary muscle cells when treated with either of the two prostaglandin analogues, while there were no changes in HTM with such treatment. Optineurin and αB-crystallin levels remained unchanged, but myocilin in the HTM cells was decreased in some samples. Conclusions: Both analogues changed gene expression similarly in either HCM or HTM cells. Down-regulated prostaglandin FP receptor in HCM when treated with prostaglandin analogues indicated that reduction of the receptor might have profound impact on long-term efficacy of such drugs. Prostaglandin analogues showed little impact on the gene associated with glaucoma in either HCM or HTM cells.
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