May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Induction of Cytokine Release after Mechanical Stress in Trabecular Meshwork Cells
Author Affiliations & Notes
  • P.B. Liton
    Ophthalmology, Duke University, Durham, NC, United States
  • M. Caballero
    Ophthalmology, Duke University, Durham, NC, United States
  • P. Gonzalez
    Ophthalmology, Duke University, Durham, NC, United States
  • D.L. Epstein
    Ophthalmology, Duke University, Durham, NC, United States
  • Footnotes
    Commercial Relationships  P.B. Liton, None; M. Caballero, None; P. Gonzalez, None; D.L. Epstein, None.
  • Footnotes
    Support  NEI RO1EY01894, P30 EY05722, Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1159. doi:
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    • Get Citation

      P.B. Liton, M. Caballero, P. Gonzalez, D.L. Epstein; Induction of Cytokine Release after Mechanical Stress in Trabecular Meshwork Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1159.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To test the hypothesis that mechanical stress induces the release of cytokines in primary cultured human trabecular meshwork (HTM) cells. Methods: Primary cultures of HTM cells at passage three were grown to confluency in Flexcell collagen coated six well plates. One week after confluency the cultures were subjected to cyclic mechanical stress in a Flexcell FX-4000T system. The stress regime consisted of cycles of 5 % stretching with a frequency of one cycle per second. Samples of the culture media were collected after 9 hours of cyclic stretching for ELISA analysis of TGF-beta-1, IL-6, IL-8, and VEGF. Viability assays were performed at the end of each experiment. ELISA analysis and viability assays were also performed in control cultures of the same cell lines. Results: ELISA Analysis shows a consistent increase of TGF-beta-1, IL-6, IL-8, and VEGF in the media of mechanically stressed HTM cultures with respect to controls after nine hours of stress. The regime of mechanical stress used did not have any measurable effect on cell viability. Conclusions:The release of cytokines from HTM cells induced by mechanical stress could play a significant role in the modulation of outflow resistance, since these molecules could potentially alter extracellular matrix composition of the TM and/or the permeability of Schlemm’s canal endothelium.

Keywords: trabecular meshwork • cytokines/chemokines • gene/expression 
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