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E. Adjadj, S. Roy, A. Mermoud; Release of Mitomycine C in a Staar Collagene Implant In Vitro Study . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1193.
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Purpose: The purpose of the study was to assess the theoretical dose that a collagene implant could contain and the cinetic of the mitomycine Methods: 4 series of 10 Staar collagene implants were soaked in a 2mg/ml mitomycine C solution for 15 minutes. Their weight was measured dry and after the soaking. Each serie was then washed in a 20 ul/min flow of distilated water. In 2 series, water was collected each hour for 24 hours to measure the time of total washing. In the two other series, water was collected at 10, 20 min, and then, each 20 minutes for 8 hours. The concentration of mitomycine was then measured with a spectrophotometer using the absorbtion at 367 nm. Results: The mean weight of a dry implant was 0.63 mg. The mean weight of of soaked implant was 2.2 mg. The mean mass of mitomycine in each implant was 3.22 ug. The concentration of mitomycine in the washing water after 10 minutes was 0.13 mg/ml. After 6 hours, mitomycine concentration was 0.05 ug/ml and was not detectable afterwards. The cinetic of the washing showed a fast diminution of the concentration in the first 60 minutes. Then the concentration stayed between 0.017 mg/ml and 0.006 mg/ml from 60 to 120 minutes. Conclusions: The mean dose of mitomycine that a collagene implant can absorb is similar to the dose used with a sponge in non penetrating filtering surgery of glaucoma. The maximum concentration obtained in vitro was 0,14 mg/ml. The concentration used with the sponge is 0.2 mg/ml.
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