May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Collagen Synthesis by Conjunctival Fibroblasts in Culture: Response to Halofuginone
Author Affiliations & Notes
  • O. Geyer
    Ophthalmology, Carmel Medical Center, Haifa, Israel
  • O. Genina
    Institute of Animal Science, The Volcani Center, Bet Dagan, Israel
  • M. Pines
    Institute of Animal Science, The Volcani Center, Bet Dagan, Israel
  • Footnotes
    Commercial Relationships  O. Geyer, None; O. Genina, None; M. Pines, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1206. doi:
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      O. Geyer, O. Genina, M. Pines; Collagen Synthesis by Conjunctival Fibroblasts in Culture: Response to Halofuginone . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1206.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Collagen deposition is largely responsible for scar formation, an undesirable outcome of glaucoma filtering surgery. Because halofuginone was found to inhibit collagen type I gene expression and collagen synthesis in animal models characterized by excessive deposition of collagen, we examined its effect on collagen synthesis from fibroblasts cultured from human conjunctiva. Methods: Human Tenon's capsule fibroblasts were obtained at the time of cataract surgery and established in individual cell cultures. Cell proliferation was measured by cell counting techniques after dissociation with trypsin/EDTA. Cell cultures were also incubated with radio-labeled proline and collagen synthesis was evaluated by digestion with collagenase. In addition, RNA isolation and Northern blot analysis were used to determine collagen alpha 1(I) gene expression. Results: Halofuginone caused a dose-dependent inhibition in fibroblast cell proliferation. Collagen dependent protein synthesis was inhibited by halofuginone with a dose-dependency. A halofuginone-induced decrease in collagen gene expression was found and it exhibited a halofuginone dose-dependent inhibition. Conclusions: Halofuginone is a novel and promising new modality to prevent collagen synthesis and scarring in glaucoma patients going through surgery.

Keywords: wound healing 
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