May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Induction of the Differentiation of Lentoids from Primate Embryonic Stem Cells
Author Affiliations & Notes
  • S. Ooto
    Ophthalmology & Vis Sci, Kyoto Univ Grad Med Sch, Kyoto, Japan
  • M. Haruta
    Experimental Therapeutics, Translational Research Center, Kyoto Univ Hospital, Kyoto, Japan
  • Y. Honda
    Experimental Therapeutics, Translational Research Center, Kyoto Univ Hospital, Kyoto, Japan
  • H. Kawasaki
    Neurobiology, Duke University Medical Center, North Carolina, NC, United States
  • Y. Sasai
    Organogenesis and Neurogenesis Group, Center for Developmental Biology,RIKEN, Kobe, Japan
  • M. Takahashi
    Organogenesis and Neurogenesis Group, Center for Developmental Biology,RIKEN, Kobe, Japan
  • Footnotes
    Commercial Relationships  S. Ooto, None; M. Haruta, None; Y. Honda, None; H. Kawasaki, None; Y. Sasai, None; M. Takahashi, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1229. doi:
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    • Get Citation

      S. Ooto, M. Haruta, Y. Honda, H. Kawasaki, Y. Sasai, M. Takahashi; Induction of the Differentiation of Lentoids from Primate Embryonic Stem Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1229.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To produce lens cells from primate embryonic stem (ES) cells in a reproducible controlled manner. Methods: Cynomologus monkey ES cells were induced to differentiate by stromal cell-derived inducing activity (SDIA). The lentoids produced by this treatment were processed for immunohistochemical and immunoblotting analysis. We also examined the effect of varying the concentration of fibroblast growth factor 2 (FGF-2) and the density of the ES colonies plated on the differentiation process. Results: Following a 2-3 week induction period, lentoids were produced by a subpopulation of ES colonies. Western blotting and immunohistochemistry revealed that these lentoids expressed crystallin alpha. The number of lentoids resulting from treatment increased with increasing FGF-2 concentration and plated colony density. Conclusions: The differentiation of primate ES cells into lentoids can be achieved by treatment with stromal cell-derived inducing activity. ES cells can be used to facilitate a greater understanding of the mechanisms functioning in differentiation in vivo and in vitro.

Keywords: crystallins • growth factors/growth factor receptors 
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