Purchase this article with an account.
S.R. Thurau, A. Fluegel, M. Diedrichs-Moehring, G. Wildner; Intraocular Reactivation of Ovalbumin-specific, GFP+ T Cells in Adoptively Transfered Immunogenic Uveitis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):722.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: In Lewis rats uveitis can be induced by adoptive transfer of CD4+ T cells and intraocular injection of their respective antigen (immunogenic uveitis). Here we wanted to investigate the fate of GFP-transduced, Ovalbumin (OVA)-specific T cells in immunogenic uveitis. Methods: OVA or bovine serum albumin (BSA) (6 µg in 10 µl) were injected into the anterior chamber of the right and left eye, immediately or 3 days after i.p. injection of GFP+ OVA-specific T cells. Eyes were examined daily for the development of clinical signs of uveitis. On day 5 eyes, lymphnodes, spleens and peripheral blood were collected. We tested GFP+ cells for expression of lymphocyte activation markers using FACS-analysis. Results: On day 4 the first clinical signs of uveitis developed only in those eyes that had received OVA at day 3 (9 of 11 eyes, avg. clinical score: 0.95). All other injected eyes remained negative. FACS-analysis revealed a marginal increase of GFP+ cells in OVA-injected compared to BSA-injected eyes. Activation makers such as OX-40 and IL-2-receptor as well as class II expression were upregulated on GFP-positive cells in uveitic, but not in healthy eyes nor in spleen, lymph nodes or peripheral blood. Conclusions: Only when immigrating T cells recognize their respective antigen within the eye they get reactivated and thus enabled to induce uveitis.
This PDF is available to Subscribers Only