May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
RPE Cells Secrete Soluble Factors Ex Vivo That Inhibit Neutrophil Activation and Prevent NK Cell-Mediated Cytotoxicity
Author Affiliations & Notes
  • P. Zamiri
    Ophthalmology, Schepens Eye Research Inst, Boston, MA, United States
  • J.W. Streilein
    Ophthalmology, Schepens Eye Research Inst, Boston, MA, United States
  • Footnotes
    Commercial Relationships  P. Zamiri, None; J.W. Streilein, None.
  • Footnotes
    Support  NIH Grant EY09595 and Massachusetts Lions Foundation
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 739. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      P. Zamiri, J.W. Streilein; RPE Cells Secrete Soluble Factors Ex Vivo That Inhibit Neutrophil Activation and Prevent NK Cell-Mediated Cytotoxicity . Invest. Ophthalmol. Vis. Sci. 2003;44(13):739.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Retinal pigment epithelial cells (RPE) are known to secrete factors that suppress T cell activation (adaptive immunity). The purpose of these experiments was to determine whether soluble factors produced by explanted retinal pigment epithelium (RPE) could also inhibit innate immune cell functions. Methods: An ex-vivo model of the posterior eyecup was produced, in which the anterior segment, lens, and retina were excised from enucleated eyes of adult C57BL/6 mice, leaving for study the sclera, choroid and a healthy monolayer of RPE. Serum free medium was added to these RPE eyecups and supernatants (SN) were removed after 24 hours and added to the following assays: (a) casein-stimulated neutrophils were cultured in vitro and their supernatants assayed for IL-1ß release, using an ELISA; (b) NK cells, obtained from spleens of BALB/c mice treated with poly I:C, were assayed for their capacity to lyse YAC-1 tumor cells. Neutralizing anti-TGF-ß antibodies were added to some cultures to determine the role of this cytokine in the observed inhibition. Results: Supernatants from RPE eyecups inhibited IL-1ß production by activated neutrophils, and this inhibition was partially relieved in the presence of anti-TGF-ß antibodies. RPE SN also significantly inhibited the capacity of NK cells to lyse YAC-1 target cells. Conclusions: RPE cells secrete soluble factors that inhibit activation of innate immune cells – neutrophils and NK cells. TGF-ß, a constituent of RPE supernatants, accounts at least in part for the inhibition of innate and adaptive immune cell activation. Thus, the RPE-dependent immune privilege of the subretinal space may involve inhibition of innate, as well as adaptive, immunity.

Keywords: immunomodulation/immunoregulation • retinal pigment epithelium • immune tolerance/privilege 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×