May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Influence of Cytokines on Levels of Transcription Factor Sp1 in Cultures of Human Corneal Fibroblasts
Author Affiliations & Notes
  • H. Nakamura
    Ophthal & Visual Science, University Illinois-Chicago, Chicago, IL, United States
  • J. Sugar
    Ophthal & Visual Science, University Illinois-Chicago, Chicago, IL, United States
  • B.Y. Yue
    Ophthal & Visual Science, University Illinois-Chicago, Chicago, IL, United States
  • Footnotes
    Commercial Relationships  H. Nakamura, None; J. Sugar, None; B.Y.J.T. Yue, None.
  • Footnotes
    Support  NIH grants EY03890, EY05628, EY01792 and RPB award
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 849. doi:
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      H. Nakamura, J. Sugar, B.Y. Yue; Influence of Cytokines on Levels of Transcription Factor Sp1 in Cultures of Human Corneal Fibroblasts . Invest. Ophthalmol. Vis. Sci. 2003;44(13):849.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Transcription factor Sp1 has been implicated in the activation or suppression of a large number of genes. The purpose of this study was to determine whether cytokines such as interleukin-1 (IL-1) α and ß can modulate the expression of Sp1 in cultures of normal human corneal fibroblasts. Methods: Early passaged human corneal fibroblasts were treated for 24 or 48 hours with cytokines including IL-1α and ß (10 ng/ml), epidermal growth factor (EGF, 15 ng/ml), fibroblast growth factor-2 (FGF-2, 20 ng/ml), and transforming growth factor ß1 and ß2 (10 ng/ml) in serum-free medium. The cells were either fixed for immunohistochemical evaluation of Sp1 level or harvested for nuclear extraction and Western blotting. Results: The Sp1 nuclear staining in corneal fibroblasts was enhanced by all the cytokines studied. The enhancement was most dramatically observed with EGF and FGF-2. Western blot analyses confirmed the immunohistochemical results. The level of Sp1 in EGF and FGF-2 treated cells was respectively, 2.9- and 2.6-fold higher compared to that in controls. Conclusions: The present study shows that the Sp1 expression in corneal fibroblasts is subjected to regulation by cytokines, especially EGF and FGF-2. This may have implications in situations with altered cytokine expression such as in wound healing or in diseases including keratoconus.

Keywords: cornea: basic science • cornea: stroma and keratocytes • transcription factors 
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