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K. Jin, U.S. Kim, T.K. Park, J.M. Kim, J.K. Park; The Effect of Air Contact on MUC1 Expression in Rabbit Corneal Epithelial Culture . Invest. Ophthalmol. Vis. Sci. 2003;44(13):853.
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Purpose: To investigate the effect of air-contact on MUC1 expression and epithelial differentiation in rabbit corneal epithelial cell culture. Methods: The rabbit corneal epithelial cells were cultured in air-contact culture system using multiwell plate membrane or conventional culture system for 5 days. The corneal epithelial cell differentiation was determined by hematoxylin-eosin stain. The MUC1 expression was determined by immunohistochemical stain using monoclonal anti-human milk fat globulin for MUC1 (HMFG-α). Results: The corneal epithelium was more differentiated in air-contact culture than conventional culture. MUC1 was detected at apical portion of air-contact cultured corneal epithelium in immunohistochemical stain, whereas there was no MUC1 staining on corneal epithelium in conventional culture. Conclusions: The air (possibly O2) influenced on the expression of MUC1 in cultured rabbit corneal epithelium and also affected the cellular differentiation of corneal epithelium
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