Purchase this article with an account.
M. Koulikovska, A. Podskochy, P. Fagerholm; Expression of the Chaperonin Containing T-complex Polypeptide 1 Chaperonin Subunit During Corneal Wound Healing . Invest. Ophthalmol. Vis. Sci. 2003;44(13):854.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: This study was designed to demonstrate the expression of the eta subunit of the hetero-oligomeric particle CCT (the chaperonin containing T-complex polypeptide 1 of TCP-1) in untreated corneas and corneas treated with ultraviolet radiation (UVR). The CCT chaperonin in the wound may promote the formation of myofibroblasts, a cell type that correlates highly with scarring in postnatal wound healing, by the folding of sufficient alpha-smooth muscle actin to form the stress fibers characteristic of these cells. Therefore, investigating of pattern of CCT chaperonin expression during corneal wound healing could be a helpful and interesting step in solving the problem of corneal scarring and loss of transparency. Methods: Rabbit corneas were exposed to 310 nm UVR at a dose producing photokeratitis (0.47 J/cm2). Corneal samples were harvested at various time points ranging from 1 to 5 days after treatment. Semi-quantitative RT-PCR was used to demonstrate CCT chaperonin gene expression. Results: Semi-quantitative RT-PCR for CCT chaperonin gene expression indicated an upregulated expression of CCT eta subunit over 1 to 2 day time period while corneas from untreated rabbits exhibited a lower and shorter expression. Conclusions: The eta subunit of CCT chaperonin was constitutively expressed in untreated rabbit corneas and was upregulated during corneal wound healing. This is in concordance with data on expression of alpha-smooth muscle actin, CCT chaperonin substrate. However, the possibility cannot be excluded that eta subunit has additional functions in the process of keratocyte transformation.
This PDF is available to Subscribers Only