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A.C. Inglis, K. Sisley, A.D. Singh, Z.I. Currie, I.G. Rennie; Prognostic Factors in Uveal Melanoma: Correlation of Vascular Loop Patterns with Genetic Imbalance as Determined Using Fluorescence in Situ Hybridization (FISH) . Invest. Ophthalmol. Vis. Sci. 2003;44(13):932.
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Purpose: To correlate vascular loop patterns with genetic imbalance between chromosomes 3 and 8 in patients with uveal melanoma. Methods: 16 patients with large choroidal or ciliary body melanomas underwent primary enucleation. Fresh tissue was harvested using standard techniques. Histopathological prognostic factors including vascular loop patterns were evaluated. These patterns were graded 1-9 using Folberg’s classification criteria (Folberg et al, Ophthalmology 100:1389-98, 1993). Genetic imbalance was determined using FISH analysis as previously described (Patel et al, BJO 2001;85: 1440-1444). Statistical significance was determined using Fisher 2 sided test. Results: 16 eyes of 16 patients were included in the study. (8 choroidal melanoma and 8 ciliary body melanoma). Vascular patterns were graded 1-9 and separated into 2 groups according to recognized prognosis. 7 patients had patterns 1-8 and 9 patients had pattern 9, which carries a poorer prognosis. Genetic imbalance of greater than 70 % between loss of chromosome 3 and gains in chromosome 8 was found in 12 patients. A statistically significant (Fisher test; pvalue = 0.02) correlation was observed between prognoses as determined by vascular loop patterns and genetic imbalance using FISH analysis. Conclusions: Vascular loop patterns correlated with genetic imbalance in this study. Larger studies are required to establish a possible association. View OriginalDownload SlideView OriginalDownload Slide
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