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P.G. FitzGerald, A. Alizadeh, J. Hess, T. Blankenship; Ultrastructural Characterization of Lenses Lacking the Lens-Specific Intermediate Filament Protein CP49 . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1254.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To deduce the function of the Beaded Filament, a lens-specific derivative of the intermediate filament family. Methods: CP49 expression was negated through targeted genomic deletion techniques. Normal lenses were examined by light and electron microscopy. To reveal the fiber cell cytoskeleton, "ghosts" of lens fiber cells were created by extraction of frozen sections, and subsequent processing for electron microscopy. Results: Negation of CP49 expression results in the absence of beaded filaments. In wild type lenses, beaded filaments show a differential relationship to the fiber cell-fiber cell interdigitations: cytoplasmic beaded filaments are absent from the concave region of at least some populations of fiber cell-fiber cell interdigitations. Conclusions: CP49 is required for beaded filament assembly. Beaded filaments may play a role in the formation of normal fiber cell-fiber cell interdigitations, a role consistent with the general function of intermediate filaments in the stabilization of the differentiated phenotype.
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