May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Ultrastructural Characterization of Lenses Lacking the Lens-Specific Intermediate Filament Protein CP49
Author Affiliations & Notes
  • P.G. FitzGerald
    Cell Biology & Human Anatomy, UC Davis School of Medicine, Davis, CA, United States
  • A. Alizadeh
    Cell Biology & Human Anatomy, UC Davis School of Medicine, Davis, CA, United States
  • J. Hess
    Cell Biology & Human Anatomy, UC Davis School of Medicine, Davis, CA, United States
  • T. Blankenship
    Cell Biology & Human Anatomy, UC Davis School of Medicine, Davis, CA, United States
  • Footnotes
    Commercial Relationships  P.G. FitzGerald, None; A. Alizadeh, None; J. Hess, None; T. Blankenship, None.
  • Footnotes
    Support  NIH Grant EY08747
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1254. doi:
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    • Get Citation

      P.G. FitzGerald, A. Alizadeh, J. Hess, T. Blankenship; Ultrastructural Characterization of Lenses Lacking the Lens-Specific Intermediate Filament Protein CP49 . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1254.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To deduce the function of the Beaded Filament, a lens-specific derivative of the intermediate filament family. Methods: CP49 expression was negated through targeted genomic deletion techniques. Normal lenses were examined by light and electron microscopy. To reveal the fiber cell cytoskeleton, "ghosts" of lens fiber cells were created by extraction of frozen sections, and subsequent processing for electron microscopy. Results: Negation of CP49 expression results in the absence of beaded filaments. In wild type lenses, beaded filaments show a differential relationship to the fiber cell-fiber cell interdigitations: cytoplasmic beaded filaments are absent from the concave region of at least some populations of fiber cell-fiber cell interdigitations. Conclusions: CP49 is required for beaded filament assembly. Beaded filaments may play a role in the formation of normal fiber cell-fiber cell interdigitations, a role consistent with the general function of intermediate filaments in the stabilization of the differentiated phenotype.

Keywords: cytoskeleton • cataract • microscopy: electron microscopy 
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