May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Cultivation of Limbal Stem Cells on Human Amniotic Membrane in the Treatment of Corneal Alkali Burns
Author Affiliations & Notes
  • R.C. Gutiérrez
    Ophthalmology, Hospital Rísquez, Caracas, Venezuela
  • O. De Marcucci
    Biochemistry, Universidad Central de Venezuela, Caracas, Venezuela
  • J. Espinoza
    Biochemistry, Universidad Central de Venezuela, Caracas, Venezuela
  • M. Orellana
    Pathology, Universidad Central de Venezuela, Caracas, Venezuela
  • A. Behrens
    Pathology, Universidad Central de Venezuela, Caracas, Venezuela
  • Footnotes
    Commercial Relationships  R.C. Gutiérrez, None; O. De Marcucci, None; J. Espinoza, None; M. Orellana, None; A. Behrens, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1361. doi:
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      R.C. Gutiérrez, O. De Marcucci, J. Espinoza, M. Orellana, A. Behrens; Cultivation of Limbal Stem Cells on Human Amniotic Membrane in the Treatment of Corneal Alkali Burns . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1361.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate the beneficial effects of three different surgical techniques to promote healing after an experimental alkali burn model in rabbits. Methods: A superficial corneal burn (NAOH 1 M) was performed in the left eye of 35 New Zealand white rabbits. Animals were divided in 4 groups (A, B and C: n=10 each, D:n=5) according to surgical treatment: Group A, preserved human amniotic membrane transplant; Group B, autologous limbal transplant covered with amniotic membrane; Group C, cultivated limbal epithelial cells on human amniotic membrane transplant; and Group D, no surgical treatment (control group). Reepitelialization progress was recorded every 72h and clinical improvement of the ocular surface analyzed with digital images. After two weeks, rabbits were sacrificed and histology of the samples was performed. Results: In the groups with surgical treatment (A, B and C), the epithelial defect was significantly smaller than controls at day 15 (P<0.002). However, no statistical differences were observed within the different surgical approaches. In the quantitative analysis of the cornea and limbal neutrophils, groups A, B and C showed less cells compared to controls (P<0.05) without significant changes in the lymphocyte population. Overall, ocular surface was qualitatively closer to normal in group C. Conclusions: Surgical intervention after a chemical burn with the studied techniques appear to be reasonable approaches in our model. Although cultivated limbal epithelial cells in amniotic membrane did not show significant differences over other techniques, it seems to be more favorable in terms of the anatomical results obtained.

Keywords: cornea: epithelium • clinical laboratory testing • anterior segment 
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