May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Effects of Povidone Iodine on Cultured Corneal Epithelial Cells
Author Affiliations & Notes
  • M. Fukuda
    Ophthalmology, Kanazawa Medical University, Uchinada, Japan
  • H. Murano
    Ophthalmology, Medical Research Institute, Kanazawa Medical University, Kanazawa, Japan
  • Y. Yamashiro
    Ophthalmology, Medical Research Institute, Kanazawa Medical University, Kanazawa, Japan
  • N. Takahashi
    Ophthalmology, Medical Research Institute, Kanazawa Medical University, Kanazawa, Japan
  • Footnotes
    Commercial Relationships  M. Fukuda, None; H. Murano , None; Y. Yamashiro , None; N. Takahashi , None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1366. doi:
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    • Get Citation

      M. Fukuda, H. Murano, Y. Yamashiro, N. Takahashi; Effects of Povidone Iodine on Cultured Corneal Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1366.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Povidone-iodine(PVP-I) is a broad-spectrum microbiocide with in vitro activity against gram-negative and gram-positive bacteria, fungi and protozoa. PVP-I is used for disinfection of palpebral skin and the conjunctival sac in ophthalmic surgery, but it induces cytotoxicity corneal tissue. In this study, the cytotoxicity of PVP-I was examined and expressed quantitatively and morphologically using cultured rabbit corneal epithelial cell lines (SIRC). Methods: We used 10% (w/v) PVP-I(Isodine ® solution) in this study. Cultured SIRC cells were exposed to PVP-I diluted at 0.312% for 30 seconds to 4 minutes, and at 0.1~0.5% for 30 seconds. The cytotoxicity of PVP-I was also microscopically (light microscopy and transmission electron microscopy ) observed after a 30 second exposure of SIRC cells to diluted PVP-I. Results: Treatment of SIRC cells with PVP-I diluted at 0.312% for 30 seconds to 4 minutes induced decrease of the cells in a treatment time-dependent manner. Exposure of cells to PVP-I at 0.1~0.5% for 30 secounds also caused decrease of the cells in a dose-dependent manner (50%viability ratio;VR-50:0.312%). On the other hand, cytotoxicity of PVP-I was microscopically observed after a 30 secound exposure of SIRC cells to PVP-I and necrosis predominated in cells treated with PVP-I solution. Conclusions: These results indicate that cytotoxicity of PVP-I occurs in dose-and treatment time-dependent manners. Microscopically, the necrosis cells predominates in cells that have been treated with PVP-I solutions. A 0.312% solution of PVP-I should be used in ocular disinfection for less than 30 seconds.

Keywords: antibiotics/antifungals/antiparasitics • cornea: epithelium • microscopy: electron microscopy 
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