May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Evaluation of the Effects of Topical Ophthalmic Fluoroquinolones (FQ) on the Cornea using In Vivo Confocal Microscopy
Author Affiliations & Notes
  • A.S. Kim
    Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, United States
  • J.P. McCulley
    Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, United States
  • H.D. Cavanagh
    Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, United States
  • J.V. Jester
    Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, United States
  • A.C. Bugde
    Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, United States
  • T. Kovoor
    Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, United States
  • W.M. Petroll
    Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, United States
  • Footnotes
    Commercial Relationships  A.S. Kim, None; J.P. McCulley, Alcon Research Ltd. R; H.D. Cavanagh, None; J.V. Jester, None; A.C. Bugde, None; T. Kovoor, None; W.M. Petroll, Alcon Research Ltd. F.
  • Footnotes
    Support  Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1367. doi:
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      A.S. Kim, J.P. McCulley, H.D. Cavanagh, J.V. Jester, A.C. Bugde, T. Kovoor, W.M. Petroll; Evaluation of the Effects of Topical Ophthalmic Fluoroquinolones (FQ) on the Cornea using In Vivo Confocal Microscopy . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1367.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To compare the effects of several FQ antibiotics on the corneal epithelium and stroma using in vivo confocal microscopy. Methods: Five antibiotic solutions were evaluated: 1) 0.3% ofloxacin (Oflox) solution with 0.005% benzalkonium chloride (BAC); 2) 0.3% gatifloxacin (Gati) solution with 0.005% BAC; 3) 0.3% ciprofloxacin (Cipro) solution with 0.006% BAC 4) 0.5% levofloxacin (Levo) with 0.005% BAC; 5) 0.5% moxifloxacin (Moxi) solution with no BAC. Tears Natural Free (Tears) was used as a control. New Zealand White rabbits were used for this study (six per solution group). Ten days prior to exposure to any solution, central corneal epithelial thickness (EpiTh) and stromal thickness (StrTh) were measured using in vivo confocal microscopy through focusing (CMTF). Images of the superficial epithelium were also acquired. Both eyes of each rabbit then received one drop of the assigned solution six times the first day and then qid for 6 days. On day 7, in vivo confocal microscopy was repeated. Results: A significant decrease in EpiTh (Table) was induced by 7 days of exposure to Levo, Gati, and Cipro. Tears and Moxi, which do not contain BAC, showed the smallest changes in EpiTh. Between group comparisons using ANOVA confirmed that Moxi induced smaller changes in EpiTh than Gati or Cipro. However, superficial epithelial morphology appeared normal in all groups. Interestingly, only the control group (Tears) showed a significant change in StrTh (Table). Conclusions: We have previously used confocal microscopy to establish a correlation between epithelial thinning (due to superficial cell loss) and slight ocular irritation (IOVS 39:2610-2625, 1998). The results of this study suggest that Moxi may induce less damage to the corneal epithelium than other antibiotic solutions, perhaps because it does not contain BAC.  

Keywords: antibiotics/antifungals/antiparasitics • ocular irritancy/toxicity testing • cornea: epithelium 
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