May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Histological Studies on Murine Ocular Heparanase
Author Affiliations & Notes
  • R.S. Berk
    Immunology & Microbiology, Wayne State University, Detroit, MI, United States
  • S. Alousi
    Immunology & Microbiology, Wayne State University, Detroit, MI, United States
  • Z. Dong
    Immunology & Microbiology, Wayne State University, Detroit, MI, United States
  • M.A. Kosir
    Immunology & Microbiology, Wayne State University, Detroit, MI, United States
  • I. Vlodavsky
    Hodassah University School of Medicine, Jerusalem, Israel
  • Footnotes
    Commercial Relationships  R.S. Berk, None; S. Alousi, None; Z. Dong, None; M.A. Kosir, None; I. Vlodavsky, None.
  • Footnotes
    Support  NIH EY11757 and PZO EY-04068
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1406. doi:
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    • Get Citation

      R.S. Berk, S. Alousi, Z. Dong, M.A. Kosir, I. Vlodavsky; Histological Studies on Murine Ocular Heparanase . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1406.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: This study was designed to investigate the expression of heparanase (ß-endoglucuronidase) in the mouse eye before and during corneal infection with Pseudomonas aeruginosa. Methods : In situ hybridization and immunohistochemical staining were used to detect and localize heparanase transcript and protein in normal mice and in mice intracorneally infected over a six day period. Results: In situ hybridization studies indicated the constitutive presence of heparanase transcript in the corneal epithelium prior to infection, but not in the stroma or endothelium. Upon infection with P. aeruginosa, the transcript was found in the stroma and associated with infiltrating cells. Immunohistochemistry of whole eyes prior to and post-infection indicated a strong correlation with the results in the cornea seen by in situ hybridization. In addition, heparanase expression was detected in most of the layers of the retina with intense staining of the inner photoreceptor layer. No staining was seen in the lens. Conclusions: These studies indicate for the first time the constitutive expression of heparanase in the cornea and retina of normal and infected murine eyes, infiltrating cells during infection and blood vessels.

Keywords: bacterial disease • immunohistochemistry • enzymes/enzyme inhibitors 
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