May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
The Effects of IL4, IL10 on the Expression of Inflammatory Cytokines and Metalloproteinase in Cultured Dermal Fibroblasts After UVB Exposure
Author Affiliations & Notes
  • T.H. Choi
    Dept of Ophthalmology, Kangdong Sacred Heart Hospital, Hallym University, Seoul, Republic of Korea
  • D. Chun
    Dept of Ophthalmology, Kangdong Sacred Heart Hospital, Hallym University, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  T.H. Choi, None; D. Chun, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1420. doi:
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      T.H. Choi, D. Chun; The Effects of IL4, IL10 on the Expression of Inflammatory Cytokines and Metalloproteinase in Cultured Dermal Fibroblasts After UVB Exposure . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1420.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate the effects of IL4, IL10 on the expression of inflammatory cytokines and metalloproteinase in cultured dermal fibroblasts after UVB(312nm) irradiation. Methods: We examined the changes of cultured normal human dermal fibroblast (NHDF) proliferation at 1, 3, 5min UVB irradiation. TNF-α and IL-1ß mRNA expression from NHDF after UVB irradiation was determined by Reverse Transcriptase Polymerase Chain Reaction(RT-PCR) and quantified by densitometer. MMP-1, MMP-3 concentration after UVB exposure was measured by ELISA. After the cells were treated with various concentrations of IL4, IL10, IL4 + IL10 (0.5-50ng/ml) and irradiated with UVB for 5min, we examined the changes of TNF-α, IL-1ß mRNA and MMP-1, MMP-3 in NHDF and culture media. Results:The changes of cell proliferation at 1, 3, 5min UVB irradiation was not statistically significant. TNF-α and IL1ß mRNA expression after UV irradiation were up-regulated at 3min & 5min exposure. The inhibition of TNF-α and IL-1ß expression was achieved at all concentrations when the cells were incubated with various concentrations of IL4, IL10, IL4 + IL10 (0.5-50ng/ml) before irradiation. The Inhibitory effects were decreased when concentration was increased. MMP-1, 3 production decreased significantly at all concentration of IL4, 10, IL4 + IL10. Furthermore, the maximal inhibitory effect was achieved at 50ng/ml with IL4 + IL10. Conclusions: we proved that UVB is a potent inducer of inflammatory cytokines such as TNF-α and IL-1ß and IL4, IL10 down-regulated these cytokines, suppressed the expression of MMP-1, MMP-3.

Keywords: inflammation • cytokines/chemokines • gene/expression 
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