May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Matrix Metalloproteinases in Human Fetal Lens Development
Author Affiliations & Notes
  • N.H. Sachdev
    Ophthalmology/Pathology, Prince of Wales Hospital and UNSW, Sydney, Australia
  • N. Di Girolamo
    Pathology, University of New South Wales (UNSW), Sydney, Australia
  • P.J. McCluskey
    Ophthalmology/Pathology, St Vincent's Hospital and UNSW Pathology, Sydney, Australia
  • M.T. Coroneo
    Ophthalmology/Pathology, St Vincent's Hospital and UNSW Pathology, Sydney, Australia
  • D. Wakefield
    Ophthalmology/Pathology, St Vincent's Hospital and UNSW Pathology, Sydney, Australia
  • Footnotes
    Commercial Relationships  N.H. Sachdev, None; N. Di Girolamo, None; P.J. McCluskey, None; M.T. Coroneo, None; D. Wakefield, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1424. doi:
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      N.H. Sachdev, N. Di Girolamo, P.J. McCluskey, M.T. Coroneo, D. Wakefield; Matrix Metalloproteinases in Human Fetal Lens Development . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1424.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To ascertain the distribution of Matrix Metalloproteinases (MMPs), Tissue Inhibitors of Matrix Metalloproteinases (TIMPs) and their substrates during the development of the human lens. Methods: Normal human fetal lenses (n=22) ranging from 10-24 weeks gestation were enucleated 4-8 hours postmortem, formalin-fixed, extracted intracapsularly, paraffin embedded and sections (4 µm) placed on TES coated glass slides for immunohistochemical analysis. Mouse anti-human monoclonal antibodies directed against MMP -1, -2, -3, -9, TIMP -1, -2, -3, fibronectin, laminin and collagen type-I and IV were applied and sections graded by masked observers. MMP presence was confirmed by ELISA and Zymogram analysis. Results: Immunohistochemical analysis revealed staining for MMP -1, -2, -3 -9, TIMP -1, -2 and -3 predominantly in the germinative zone, transitional zone and posterior basement membrane complex at all gestational ages within the fetal lens. The staining pattern varied significantly over the 14-week gestational period and correlated with localized developmental stages such as suture formation, germinative epithelium migration, tunica vasculosa lentis regression and zonule formation. Conclusion: The spatial changes in expression of proteinases and their inhibitors in the fetal lens from 10-24 weeks suggests they may play a number of different roles in fetal lens remodelling and growth.

Keywords: plasticity • proteolysis • immunohistochemistry 
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