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N.H. Sachdev, N. Di Girolamo, P.J. McCluskey, M.T. Coroneo, D. Wakefield; Matrix Metalloproteinases in Human Fetal Lens Development . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1424.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To ascertain the distribution of Matrix Metalloproteinases (MMPs), Tissue Inhibitors of Matrix Metalloproteinases (TIMPs) and their substrates during the development of the human lens. Methods: Normal human fetal lenses (n=22) ranging from 10-24 weeks gestation were enucleated 4-8 hours postmortem, formalin-fixed, extracted intracapsularly, paraffin embedded and sections (4 µm) placed on TES coated glass slides for immunohistochemical analysis. Mouse anti-human monoclonal antibodies directed against MMP -1, -2, -3, -9, TIMP -1, -2, -3, fibronectin, laminin and collagen type-I and IV were applied and sections graded by masked observers. MMP presence was confirmed by ELISA and Zymogram analysis. Results: Immunohistochemical analysis revealed staining for MMP -1, -2, -3 -9, TIMP -1, -2 and -3 predominantly in the germinative zone, transitional zone and posterior basement membrane complex at all gestational ages within the fetal lens. The staining pattern varied significantly over the 14-week gestational period and correlated with localized developmental stages such as suture formation, germinative epithelium migration, tunica vasculosa lentis regression and zonule formation. Conclusion: The spatial changes in expression of proteinases and their inhibitors in the fetal lens from 10-24 weeks suggests they may play a number of different roles in fetal lens remodelling and growth.
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