May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Experimental Mycobacteria Infection in Keratomileusis
Author Affiliations & Notes
  • C.B. Diniz Adan
    Ophthalmology, Federal School of Medicine, São Paulo, Brazil
  • E.H. Sato
    Ophthalmology, Federal School of Medicine, São Paulo, Brazil
  • L.B. Sousa
    Ophthalmology, Federal School of Medicine, São Paulo, Brazil
  • R. Oliveira
    Microbiology, Federal School of Medicine, São Paulo, Brazil
  • S.C. Leão
    Microbiology, Federal School of Medicine, São Paulo, Brazil
  • A.L. Lima
    Microbiology, Federal School of Medicine, São Paulo, Brazil
  • D. Freitas
    Ophthalmology, Federal School of Medicine of São Paulo, São Paulo, Brazil
  • Footnotes
    Commercial Relationships  C.B. Diniz Adan, None; E.H. Sato, None; L.B. Sousa, None; R. Oliveira, None; S.C. Leão, None; A.L.H. Lima, None; D. Freitas, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1426. doi:
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      C.B. Diniz Adan, E.H. Sato, L.B. Sousa, R. Oliveira, S.C. Leão, A.L. Lima, D. Freitas; Experimental Mycobacteria Infection in Keratomileusis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1426.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To develop an experimental animal model of infection by M. chelonae in keratomileusis. Methods: Double masked, prospective study consisting of 2 experiments, each one with 8 adult male New Zealand rabbits. Eight eyes of 8 rabbits underwent uneventful partial keratomileusis with nasal hinge by the automatic corneal shaper (ACS® by Bausch&Lomb) under general anesthesia. Every eye was immunossupressed by local injection of methyl prednisolone. Controls: 4 animals underwent left keratomileusis. Inoculum consisting of 1 microliter of 107 heat-inactivated bacteria was inoculated in the interface<strike>s</strike> . Subjects: 4 animals underwent exactly to the same procedure as control. Inoculum consisted of 1 microliter of 107.active bacteria. Trimethoprim 0.1% drops were administrated each 4 hours as antibiotic prophylaxis. Animals were examined by 2 observers on slit lamp on the 1st , 3 rd, 5th, 7th, 11th and 16th post-operative days. Slit lamp photos were taken to evaluate clinical signs. Animals were sacrificed when corneal disease emerged. Cornea samples were taken for microbiologic studies. Results: First series: three control rabbits did not present corneal disease. One presented secondary infection. Subjects: three subject rabbits developed corneal disease and we were able to isolate M. chelonae. Second series: None of control rabbits developed clinical disease and four of subject rabbits developed corneal disease. We were able to isolate M. chelonae in three of subject rabbits. Conclusions:Corneal infection by M. chelonae has been succesfully induced in rabbits undergoing keratomileusis.

Keywords: animal model • bacterial disease • keratitis 
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