May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Detection of Bacteria on Ocular Infections by Nested PCR in Mexico City
Author Affiliations & Notes
  • M.A. Reyes-Lopez
    Microbiology, Instituto de Oftalmologia, Mexico City, Mexico
  • J.C. Cancino-Díaz
    Microbiology, Instituto de Oftalmologia, Mexico City, Mexico
  • S. Muñoz-Salas
    Microbiology, Instituto de Oftalmologia, Mexico City, Mexico
  • M. Sánchez
    Microbiology, Instituto de Oftalmologia, Mexico City, Mexico
  • O. Niño-de-Rivera
    Microbiology, Instituto de Oftalmologia, Mexico City, Mexico
  • A. Climent-Flores
    Microbiology, Instituto de Oftalmologia, Mexico City, Mexico
  • Footnotes
    Commercial Relationships  M.A. Reyes-Lopez, None; J.C. Cancino-Díaz, None; S. Muñoz-Salas, None; M. Sánchez, None; O. Niño-de-Rivera, None; A. Climent-Flores, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1432. doi:
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    • Get Citation

      M.A. Reyes-Lopez, J.C. Cancino-Díaz, S. Muñoz-Salas, M. Sánchez, O. Niño-de-Rivera, A. Climent-Flores; Detection of Bacteria on Ocular Infections by Nested PCR in Mexico City . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1432.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To establish the most common infection and isolated bacteria and to detect and to discriminate between Gram-positive and Gram-negative bacteria by nested PCR on ocular infections. Methods: The samples were isolated from patients with ocular infections. Bacterial frequency of last five years was collected and analyzed. Bacteria were confirmed by Gram staining, isolated, and plated. Bacteria were identified using a biochemical identification automatic system. To establish the molecular study, forty eight conjunctivas, two contact lenses, and one intraocular (vitreous) samples were taken. Nested and multiplex PCR of universal and specific regions for Gram-positive bacteria were performed. Also, a single PCR reaction was done for detecting Gram-negative. On another hand, nested PCRs were performed to identify fungus. Results: One percent (1200) of all patients who came to the Institute was analyzed. From those data, 77% were conjunctivitis, 12% were ulcers, 3% were endopthalmitis, and 8% others. From most common fifteen isolated bacteria, Staphylococcus epidermidis and Staphylococcus aureus were on conjunctiva. Time to perform the PCR's reactions was lower than cultures, four hours for PCR and between 24 and 48 hours for culture. Conclusion: The most common infection and isolated bacteria in around five years were detected and identified on ocular infections. On other hand, due the speed and accuracy, detection of bacteria by PCR will help and have great impact in ocular infections' analysis in Mexico.

Keywords: bacterial disease • clinical (human) or epidemiologic studies: bio • conjunctivitis 
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