Purchase this article with an account.
M.A. Reyes-Lopez, J.C. Cancino-Díaz, S. Muñoz-Salas, M. Sánchez, O. Niño-de-Rivera, A. Climent-Flores; Detection of Bacteria on Ocular Infections by Nested PCR in Mexico City . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1432.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To establish the most common infection and isolated bacteria and to detect and to discriminate between Gram-positive and Gram-negative bacteria by nested PCR on ocular infections. Methods: The samples were isolated from patients with ocular infections. Bacterial frequency of last five years was collected and analyzed. Bacteria were confirmed by Gram staining, isolated, and plated. Bacteria were identified using a biochemical identification automatic system. To establish the molecular study, forty eight conjunctivas, two contact lenses, and one intraocular (vitreous) samples were taken. Nested and multiplex PCR of universal and specific regions for Gram-positive bacteria were performed. Also, a single PCR reaction was done for detecting Gram-negative. On another hand, nested PCRs were performed to identify fungus. Results: One percent (1200) of all patients who came to the Institute was analyzed. From those data, 77% were conjunctivitis, 12% were ulcers, 3% were endopthalmitis, and 8% others. From most common fifteen isolated bacteria, Staphylococcus epidermidis and Staphylococcus aureus were on conjunctiva. Time to perform the PCR's reactions was lower than cultures, four hours for PCR and between 24 and 48 hours for culture. Conclusion: The most common infection and isolated bacteria in around five years were detected and identified on ocular infections. On other hand, due the speed and accuracy, detection of bacteria by PCR will help and have great impact in ocular infections' analysis in Mexico.
This PDF is available to Subscribers Only