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T. Tochikubo, M. Kimura, S. Kobayakawa, Y. Okajima, Y. Katayama, A. Tsuji; In vitro Adherence to Intraocular Lenses and Hemolysin of Enterococcus faecalis Strains . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1437.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: E. faecalis which has the beta-hemolysin is strong pathogenicity. We investigated whether the presence of the beta-hemolysin affected the adherence to intraocular lenses (IOL) in vitro. Methods: Ten E. faecalis isolates from stools of patients were investigated.1.Five beta-hemolysin strains and five non-hemolysin were used. One-piece PMMA IOLs were placed in different test plate containing E. faecalis suspension (10(5) cfu/ml) and cultured for 24 hours. Quantitative cultures were performed.2.Adherence of one beta-hemolysin strain and one non-hemolysin were tested on one-piece(1P) PMMA, 1P and three-piece(3P) silicone, 1P and 3P acrylic IOLs. At different times (1,24, and 48 hours), each IOL was separated the adhered bacteria. Quantitative cultures were performed. Results: 1.The mean pre-culture bacterial suspension of beta-hemolysin strains was 4.14 (cfu/IOL,log), the mean adherence was 3.39. The mean pre-culture suspension of non-hemolysin was 4.93, the mean adherence was 5.67. 2.The mean pre-culture bacterial suspension of beta-hemolysin strain was 5.69, non-hemolysin was 5.49. After 1,24,48 hours, the mean adherence to IOLs of beta-hemolysin was 0, 3.34 and 4.14. The mean of non-hemolysin was 1.44, 3.76 and 5.43. The IOL materials affected the adherence. Non-hemolysin strain was more adhesive to 3p-silicone, 1p and 3p-acrylic IOLs. There were no difference to 1p-silicone between both strains. Conclusions: The beta-hemolysin strains of E. faecalis were not more adhesive to IOLs than the non-hemolysin.
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