May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Determine the In-vitro Antimicrobial Preservative Effectiveness of a New Broad Spectrum Antimicrobial Preservative "Oxyd"
Author Affiliations & Notes
  • V.H. Karageszian
    Ophthalmology, UC Irvine, Irvine, CA, United States
  • H.L. Karageozian
    S.K. Pharmaceuticals, Inc, San Juan Capistrano, CA, United States
  • A. Climent
    Ophthalmology, Instituto de Oftalmologia conde de Valenciana, Mexico City, CA, United States
  • S. Munoz
    Ophthalmology, Instituto de Oftalmologia Conde de Valenciana, Mexico City, CA, United States
  • G. Cuevas
    Ophthalmology Research Laboratory, Laboratorio Sophia, Guadalajara, Mexico, CA, United States
  • V.G. Gallegos
    Ophthalmology Research Laboratory, Laboratorios Sophia, Guadalajara, Mexico, CA, United States
  • Footnotes
    Commercial Relationships  V.H. Karageszian, S.K. Pharmaceutical, Inc C; H.L. Karageozian, S.K. Pharmaceuticals, Inc. P; A. Climent, None; S. Munoz, None; G. Cuevas, None; V.G. Gallegos, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1469. doi:
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      V.H. Karageszian, H.L. Karageozian, A. Climent, S. Munoz, G. Cuevas, V.G. Gallegos; Determine the In-vitro Antimicrobial Preservative Effectiveness of a New Broad Spectrum Antimicrobial Preservative "Oxyd" . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1469.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate the in-vitro antimicrobial preservativeeffectiveness of a new broad spectrum antimicrobial preservative"Oxyd" in an Ophthalmic tear solution.Methods: The preservativeeffectiveness test methodology of the United States Pharmacopeia25 (USP) was used in all the in-vitro testing Working culturesof ATCC strains of P.aeruginosa, S.aureus, E.coli, C. albicansand A. niger, or human resistant strains were prepared as perthe USP. Sterile saline suspensions of the five organisms areprepared in separate sterile culture tubes, to contain 106 -108 colony forming units/ mL. of solution. 20 mL. of productsample is transferred aseptically to 5 sterile capped bacteriologicaltubes, Each product tube is inoculated with one of the standardizedmicrobial suspensions, using a ratio equivalent to 0.10 mL.of inoculum to 20 mL. of product. and mixed. The number of viablemicroorganisms in each inoculum suspension is determined bythe plate-count method. The inoculated containers are incubatedat 20° - 25°. The culture tubes are read at 1, 2, 3,4, 6 and 24 hours,7 and 14 days.Results: 

 Conclusions: "Oxyd" the new antimicrobial preservative is veryeffective as a broad spectrum antimicrobial agent.

Keywords: antibiotics/antifungals/antiparasitics • cornea: tears/tear film/dry eye • bacterial disease 
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