May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Effects of Interleukin-1 on the Growth and Melanogenesis of Human Uveal Melanocytes In Vitro
Author Affiliations & Notes
  • S.N. Mehta
    Dept Pathology, New York Eye & Ear Infirmary, New York, NY, United States
  • D. Hu
    Dept Pathology, New York Eye & Ear Infirmary, New York, NY, United States
  • S.A. McCormick
    Dept Pathology, New York Eye & Ear Infirmary, New York, NY, United States
  • Footnotes
    Commercial Relationships  S.N. Mehta, None; D. Hu, None; S.A. McCormick, None.
  • Footnotes
    Support  New York Eye and Ear Infirmary Departments of Ophthalmology and Pathology Research Funds, New York,
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1557. doi:
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      S.N. Mehta, D. Hu, S.A. McCormick; Effects of Interleukin-1 on the Growth and Melanogenesis of Human Uveal Melanocytes In Vitro . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1557.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To study the effect of Interleukin-1 (IL-1) and Interleukin-6 (IL-6) on the growth and melanogenesis of cultured uveal melanocytes (UM). Methods: Human UM were isolated and cultured from donor eyes as previously reported. Early passages of UM were plated into multi-well plates and cultured with complete medium (F12 medium with 20 ng/ml bFGF, 1 mM IBMX, 10 ng/ml cholera toxin and 10% serum). IL-1α or IL-6 was added to the culture medium at various concentrations. Each group was performed in triplicate. Cells cultured with the complete medium without ILs were used as controls. After 6 days, cells were detached and counted. Melanin content was measured spectrophotometrically. Results: UM cultured with complete medium grew well and produced melanin in vitro. Both IL-1α and IL-6 significantly inhibited growth and melanogenesis in a dose-dependent manner (IL-1 α, 0.01-10 ng/ml; IL-6, 10-300 ng/ml). The cell number of IL-1α and IL-6 treated groups was 52% (IL-1α, 10 ng/ml) and 72% (IL-6, 100 ng/ml) of the controls, respectively. Conclusions: It has been reported that some inflammatory mediators (e.g. Prostaglandin E) stimulate the growth and melanogenesis of UM in vitro. However, the UM in vivo do not respond to inflammation or immune reaction. The growth inhibiting effect of ILs may counteract the stimulating effect of PGE or other stimulatory mediators to maintain the stability of UM observed in vivo during inflammatory events.

Keywords: melanocytes • inflammation 
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