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S.S. Zhang, X. Xu, C.J. Barnstable, X. Fu; Genome-wide Specification of Retina Transcripts During Mouse Retina Development . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1593.
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Purpose: Tissue-specific and enriched transcripts may contribute to tissueor cell identities during development and to functional processes.Potentially, alteration in these transcripts could lead to disease in the specific organ or tissue. The retina develops from the central nervous system (CNS) and is often used as a model for studying CNS development. It is susceptible to a variety of diseases or genetic alterations that can lead to vision loss or complete blindness. A dynamic process of cell differentiation occurs during development in mouse retina from embryonic day 12 to postnatal first three weeks making this a perfect model to study gene specificity. Methods: With the availability of almost complete genome and transcript databases, we have classified a set of genes with highly retina specific expression by a sequence-based gene screening integrated computational and retina tissue-specific microarray approaches during mouse development. Results: After a series of computational protocols that we have generated for EST purification and classification, about 34,000 clean ESTs were identified as retina transcripts from more than 80,000 mouse retina ESTs. By comparison with 2 million mouse ESTs from other tissue libraries, a spectrum of retina specific or enriched genes have been labeled with different grades set by reference to a known retina specific gene set. About 600 genes were characterized as the highest grade of retina specificity and these covered about 80 % of known photoreceptor disease genes. A series of microarray analyses from mouse embryonic stages to adult has been taken and compared with bioinformatics results. Conclusions: Genome-wide developmental distribution and functional clusters of the transcripts by classifying with retina tissue specificity could provide a tool to clone specific genes during retina development and pathogenesis.
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