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P.J. Linser, T.P. Moroz, M.F. Clamp, J.D. Ochrietor; Developmental Analyses of the Expression of 5A11/Basigin and 5A11/Basigin2 in the Mouse Neural Retina and RPE . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1612.
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Purpose: 5A11/Basigin is an integral membrane protein whose presence and function is crucial for vision. A knockout of the gene results in failure in the maturation of retinal function and subsequent retinal degeneration. There are two splice variants of this member of the IGSF expressed in the retina. In this study we have examined and compared the expression patterns for both 5A11/Basign and 5A11/Basigin2 in the developing mouse eye to help understand the relative importance of each variety in visual function. Methods: Mice were bred and successful matings noted via the vaginal plug method. At various time points during development in utero and post natally, mice were sacrificed. Eyes were removed and retinal expression of 5A11/Basigin and 5A11/Basigin 2 were analyzed by real-time PCR or by confocal immunohistochemistry. Results: Real-time PCR analyses of mRNA expression showed that both splice variants of 5A11/Basigin are detectable in the neural retina and RPE by embryonic day 15 in the mouse. Thereafter, levels remain low until postnatal day 7 when both exhibit substantially higher expression. The long form (5A11/Basigin2) then increases sharply in comparison to the shorter form. By postnatal day 28, the long form expression begins to drop to a level equivalent with that of the short form. Thereafter, the two forms exhibit constant and parallel expression levels. Immunohistochemical analyses show that there is low level but generalized expression of in emerging neuroblasts and glioblasts early which gradually gives way to the adult pattern: photoreceptor-specific expression of 5A11/Basigin2 with more generalized (Mhller cell and RPE) expression of 5A11/Basigin (short form). Conclusions: The two forms of 5A11/Basigin show dynamic and different patterns of expression in the eye with highest levels during the final developmental period, the postnatal days leading up to eye opening. These findings are consistent with the hypothesis that the two forms of 5A11/Basigin are distinguishable in distribution and thus possibly in function. Future studies will focus on the relative importance and rolls of each in retina development.
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