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M. Chun, E. Lee, I. Kim, W. Kang, S. Oh; Differential Expression and Cellular Localization of Doublecortin in the Developing Rat Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1614.
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Purpose: To investigate the expression and cellular localization of doublecortin (DCX) in the developing rat retina.Methods: Retinas were removed from developing rat retina. Immunohistochemistry and western blot analysis were applied to identify expression and cellular localization of DCX. Doulbe labeling using anti-DCX and ß-tubulin or calbindin antisera were applied.Results:The expression level of doublecortin was high at embryonic day (E) 18 to E 20 and became low at postnatal period. The doublecortin immunoreactivity first appeared in a few radially orientated cells in the mantle zone of the primitive retina at embryonic day 15 (E15). From E16 onward, the immunoreactivity appeared in two different regions, inner part of the retina and middle of the neuroblastic layer. In the inner part, the somata of cells in the ganglion cell layer and in the distal row of the neuroblastic layer and profiles in the inner plexiform layer showed DCX immunoreactivity up to postnatal day 1 (P1). Afterwards, the doublecortin immunoreactivity persited in the inner plexiform layer up to P15, although the intensity gradually decreased with the maturation of the retina. In the middle of the neuroblastic layer, DCX immunoreactivity appeared in the radially oriented cells. These cells transformed into horizontal cells. The doublecortin immunoreactivity persisted in these cells up to P21. Conclusions: These results suggest that doublecortin may play an important role in the migration and differentiation of specific neuronal population in developmental stages of the rat retina.
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