Purchase this article with an account.
E. Lim, S. Park, S. Oh, C. Park, J. Chung, M. Chun; Increased Expression of Ciliary Neurotrophic Factor and the Signal Transducers and Activators of Transcription (STAT3) in the Developing FVB/N Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1615.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the regulatory expression of ciliary neurotrophic factor (CNTF) and the signal transducers and activators of transcription (STAT3) in the retinal degeneration (rd) mouse. Methods: Retinas were removed from FVB/N mice at postnatal day (P) 12, 14, 16, 18, 21. Immunohistochemistry and western blot analysis were applied to identify expression and cellular localization of CNTF and STAT3. Results: In the normal developmental retina, CNTF immunoreactivity first appeared in Müller cells at P14, and then disappeared by P18. In the FVB/N retina, CNTF immunoreactivity was also appeared in Müller cells at P14, but its intensity was stronger. The immunoreactivity was continuously expressed up to P21. In the normal developmental retina, STAT3 immunoreactivity increase in Müller cells up to P16, and then disappeared. In the FVB/N retina, STAT3 immunoreactivity was also continuously expressed up to P21. Also CNTF, STAT3 quantitative evaluation by immunoblotting appear simialar pattern. Conclusions: These results suggest that Müller cells may play protective roles in the degenerative processes of FVB/N mouse via increased expression of endogenous neuroprotective system.
This PDF is available to Subscribers Only