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Y. Jiang, S. He, M. Jin, S.J. Ryan, D.R. Hinton; Oxidant Stress Inhibits PDGF-Induced RPE Migration . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1633.
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Purpose: Retinal pigment epithelial cells (RPE) undergo functional and morphological changes in response to oxidant stress. This study sought to determine if platelet derived growth factor (PDGF)-induced RPE migration could be modulated by oxidant stress induced by tert-butyl hydroperoxide (tbh). Methods: Cell migration was tested using passage 2-4 human RPE cells in a modified Boyden Chamber Assay. Cells were treated with different concentrations of tbh (10-200uM) for one hour, and chemotaxis toward PDGF (30ng/ml) was measured after an additional 5 hours. Changes in the actin cytoskeleton were evaluated microscopically with phylloidin staining. Effects of tbh on RPE cell survival were determined using the MTT assay and trypan blue staining. Results:: RPE cell migration induced by PDGF was significantly inhibited by pretreatment with tbh at a dose of 50uM and above. F-actin was aggregated in the periphery of the cells after treatment with tbh at a dose higher than 100uM. MTT assay and trypan blue staining showed no evidence of cell death during the 6 hours of this assay. When cells were maintained for 16 hours, cell death occurs with % survival ranging from 95.74 % (50 uM) to 44.44% (200 uM). Suprisingly, PDGF protected RPE cells from tbh (50 uM) -induced cell death. Conclusions: Oxidant stress induced with tbh can inhibit PDGF-induced RPE cell migration. CR: None
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