May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Oxidant Stress Inhibits PDGF-Induced RPE Migration
Author Affiliations & Notes
  • Y. Jiang
    Ophthalmology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • S. He
    Ophthalmology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • M. Jin
    Ophthalmology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • S.J. Ryan
    Ophthalmology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • D.R. Hinton
    Pathology, Doheny Vision Research Ctr, Los Angeles, CA, United States
  • Footnotes
    Commercial Relationships  Y. Jiang, None; S. He, None; M. Jin, None; S.J. Ryan, None; D.R. Hinton, None.
  • Footnotes
    Support  NIH grants EY02061,EY03040;Unrestricted grant from RPB; Arnold Mabel Beckman Foundation
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1633. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Y. Jiang, S. He, M. Jin, S.J. Ryan, D.R. Hinton; Oxidant Stress Inhibits PDGF-Induced RPE Migration . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1633.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Retinal pigment epithelial cells (RPE) undergo functional and morphological changes in response to oxidant stress. This study sought to determine if platelet derived growth factor (PDGF)-induced RPE migration could be modulated by oxidant stress induced by tert-butyl hydroperoxide (tbh). Methods: Cell migration was tested using passage 2-4 human RPE cells in a modified Boyden Chamber Assay. Cells were treated with different concentrations of tbh (10-200uM) for one hour, and chemotaxis toward PDGF (30ng/ml) was measured after an additional 5 hours. Changes in the actin cytoskeleton were evaluated microscopically with phylloidin staining. Effects of tbh on RPE cell survival were determined using the MTT assay and trypan blue staining. Results:: RPE cell migration induced by PDGF was significantly inhibited by pretreatment with tbh at a dose of 50uM and above. F-actin was aggregated in the periphery of the cells after treatment with tbh at a dose higher than 100uM. MTT assay and trypan blue staining showed no evidence of cell death during the 6 hours of this assay. When cells were maintained for 16 hours, cell death occurs with % survival ranging from 95.74 % (50 uM) to 44.44% (200 uM). Suprisingly, PDGF protected RPE cells from tbh (50 uM) -induced cell death. Conclusions: Oxidant stress induced with tbh can inhibit PDGF-induced RPE cell migration. CR: None

Keywords: age-related macular degeneration • oxidation/oxidative or free radical damage • retinal pigment epithelium 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×