May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Identification of Genes Expressed by Oxidative Stress in Retinal Pigment Epithelial Cells
Author Affiliations & Notes
  • S. Matsuda
    Ophthalmology, Osaka Univ Grad Sch Med, Suita, Japan
  • F. Gomi
    Ophthalmology, Osaka Univ Grad Sch Med, Suita, Japan
  • T. Katayama
    Anatomy and Neuroscience, Osaka Univ Grad Sch Med, Suita, Japan
  • M. Tohyama
    Anatomy and Neuroscience, Osaka Univ Grad Sch Med, Suita, Japan
  • Y. Tano
    Anatomy and Neuroscience, Osaka Univ Grad Sch Med, Suita, Japan
  • Footnotes
    Commercial Relationships  S. Matsuda, None; F. Gomi, None; T. Katayama, None; M. Tohyama, None; Y. Tano, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1634. doi:
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      S. Matsuda, F. Gomi, T. Katayama, M. Tohyama, Y. Tano; Identification of Genes Expressed by Oxidative Stress in Retinal Pigment Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1634.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To isolate the genes whose expression increases in retinal pigment epithelial (RPE) cells due to oxidative stress. Methods: Subconfluent cultures of ARPE-19 cells were treated with hypoxia/re-oxygenation or paraquat to induce oxidative stress. The mRNA was isolated from cells, and genes were screened whose expression increased due to oxidative stress using a cDNA subtraction method. The change of expression of candidate genes was confirmed by Northern blotting or reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of several genes was also analyzed in mice treated with ocular focal illumination after intraperitoneal injection of Rose Bengal photosensitive dye as a model for ocular responses to oxidative stress by RT-PCR. Results: The up-regulation of 10 genes in response to oxidative stress was confirmed and included connective tissue growth factor (CTGF) and matrix metalloproteinase-2 (MMP-2). The peak expression of MMP-2 transcripts occurred at an earlier period after treatment with oxidative stress compared with CTGF. The induction of CTGF and MMP2 transcripts also occurred in the eyes of mice treated with Rose Bengal and light. Conclusions: The expression of CTGF and MMP-2 mRNA increased in the RPE cells and in vivo after treatment with oxidative stress. Further analysis may provide a better understanding of the pathogenesis of age-related macular degeneration.

Keywords: oxidation/oxidative or free radical damage • retinal pigment epithelium • age-related macular degeneration 
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