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S. Park, E. Lim, S. Oh, C. Park, J. Chung, M. Chun; Sprouting of Glycinergic Cell Processes into the Outer Plexiform Layer in the Developing FVB/N Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1655.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To identify whether there is transneuronal morphological changes of amacrine cells, which receive indirect synaptic inputs from photoreceptor cells via bipolar cells in the developing FVB/N mouse retina. Methods: Vibratome and wholemount retinae taken from FVB/N mice at postnatal day (P) 12, 14, 16 and 21 were immunostained with antisera against glycine transporter-1 (GLYT-1). Double labeling immunohistochemistry was performed with antisera against GLYT-1 and neurofilament Hp-200 (NHp-200) or tyrosine hydroxylase (TH). Western blot analysis was also performed to evaluate GLYT-1 protein quantity. Results: From P14, a few processes of gycinergic cells ascended into the outer plexiform layer (OPL) and at P21 more ascending brush type processes were visible in the OPL. Co-localization of GLYT-1 and NHp-200 or TH within ascending processes was never observed. The GLYT-1 protein quantity increased from P14 up to P21. Conclusions: Our results suggest that glycinergic cells respond to photoreceptor degeneration via sprouting their processes into the OPL as they mature.
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