May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Sprouting of Glycinergic Cell Processes into the Outer Plexiform Layer in the Developing FVB/N Mouse Retina
Author Affiliations & Notes
  • S. Park
    Anatomy, Catholic Univ of Korea, College of Medicine, Seoul, Republic of Korea
  • E. Lim
    Anatomy, Catholic Univ of Korea, College of Medicine, Seoul, Republic of Korea
  • S. Oh
    Anatomy, Catholic Univ of Korea, College of Medicine, Seoul, Republic of Korea
  • C. Park
    Ophthalmology, Catholic Univ of Korea, College of Medicine, Seoul, Republic of Korea
  • J. Chung
    Ophthalmology, Catholic Univ of Korea, College of Medicine, Seoul, Republic of Korea
  • M. Chun
    Ophthalmology, Catholic Univ of Korea, College of Medicine, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  S. Park, None; E. Lim, None; S. Oh, None; C. Park, None; J. Chung, None; M. Chun, None.
  • Footnotes
    Support  Korea Research Foundation Grant 2001, FP0005
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1655. doi:
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      S. Park, E. Lim, S. Oh, C. Park, J. Chung, M. Chun; Sprouting of Glycinergic Cell Processes into the Outer Plexiform Layer in the Developing FVB/N Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1655.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To identify whether there is transneuronal morphological changes of amacrine cells, which receive indirect synaptic inputs from photoreceptor cells via bipolar cells in the developing FVB/N mouse retina. Methods: Vibratome and wholemount retinae taken from FVB/N mice at postnatal day (P) 12, 14, 16 and 21 were immunostained with antisera against glycine transporter-1 (GLYT-1). Double labeling immunohistochemistry was performed with antisera against GLYT-1 and neurofilament Hp-200 (NHp-200) or tyrosine hydroxylase (TH). Western blot analysis was also performed to evaluate GLYT-1 protein quantity. Results: From P14, a few processes of gycinergic cells ascended into the outer plexiform layer (OPL) and at P21 more ascending brush type processes were visible in the OPL. Co-localization of GLYT-1 and NHp-200 or TH within ascending processes was never observed. The GLYT-1 protein quantity increased from P14 up to P21. Conclusions: Our results suggest that glycinergic cells respond to photoreceptor degeneration via sprouting their processes into the OPL as they mature.

Keywords: retinal degenerations: cell biology • amacrine cells • plasticity 
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