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F.A. Soto Leon, D.M. Chacko, J. James, A.V. Das, X. Zhao, W.B. Thoreson, I. Ahmad; Lysophosphatidic Acid (LPA) Promotes Proliferation and Survival of Retinal Stem Cells/Progenitors . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1673.
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Purpose: Self-renewal and differentiation of retinal stem cells/progenitors depend on extrinsic factors. One such factor could be LPA, a small phospholipid with growth promoting activity. The purpose of this study is to determine whether or not LPA influences proliferation and differentiation of retinal stem cells/progenitors. Methods: PN1 retina cells were isolated and cultured as previously described (Ahmad et al, Brain Res. 831:1-2,1999). To evaluate the effect of LPA on cell proliferation, retinal cells were cultured in the presence of 1%FBS+LPA or LPA for 48 hours. Cells were exposed to BrdU for the final 24 hours. The proliferative response of cells was assayed by CyQuant proliferation assay kit and incorporation of BrdU. To evaluate the effect of LPA on differentiation PN1 neurospheres were cultured in the presence of 1% FBS+LPA or 1% FBS alone, followed by immunocytochemical analysis of cell specific markers. Viability of cells was monitored during proliferation and differentiation. Results: Both the number and viability of cells increased in the presence of LPA. The proportion of BrdU positive cells increased in the presence of LPA suggesting that the increase in cell number was not due to improved viability alone but reflected LPA influence on cell proliferation. When stem cells/progenitors were exposed to LPA during differentiation, the proportion of cells expressing cell specific markers decreased. Conclusions: Preliminary results suggest that LPA may play a role in the proliferation and survival of retinal stem cells/progenitors. The effect on differentiation may be secondary to its influence on cell proliferation.
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