May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
In vivo Activation of Cell Proliferation in Postnatal Mammalian Retina and Ciliary Epithelium
Author Affiliations & Notes
  • X. Zhao
    Ophthalmology, University Nebraska Med Ctr, Omaha, NE, United States
  • A.V. Das
    Ophthalmology, University Nebraska Med Ctr, Omaha, NE, United States
  • S. Edakkot
    Ophthalmology, University Nebraska Med Ctr, Omaha, NE, United States
  • J. James
    Ophthalmology, University Nebraska Med Ctr, Omaha, NE, United States
  • S. Bhattacharya
    Ophthalmology, University Nebraska Med Ctr, Omaha, NE, United States
  • F.A. Soto-Leon
    Ophthalmology, University Nebraska Med Ctr, Omaha, NE, United States
  • I. Ahmad
    Ophthalmology, University Nebraska Med Ctr, Omaha, NE, United States
  • Footnotes
    Commercial Relationships  X. Zhao, None; A.V. Das, None; S. Edakkot, None; J. James, None; S. Bhattacharya, None; F.A. Soto-Leon, None; I. Ahmad, None.
  • Footnotes
    Support  NEI and Nebraska Research Initiative
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1683. doi:
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      X. Zhao, A.V. Das, S. Edakkot, J. James, S. Bhattacharya, F.A. Soto-Leon, I. Ahmad; In vivo Activation of Cell Proliferation in Postnatal Mammalian Retina and Ciliary Epithelium . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1683.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Neural stem cells/progenitors isolated from embryonic retina and adult ciliary epithelium proliferate in vitro in the presence of EGF and/or FGF2. We are studying the ability of exogenous growth factors to promote cell proliferation in vivo in order to determine whether or not regeneration is a possibility in postnatal mammalian retina. Methods: Postnatal day 7 (PN7) rats were anesthetized and growth factors (insulin 1µg/µl, FGF2 20ng/µl) and BrdU (1µg/µl) were delivered into right eye by intraocular injection using a glass micropipette attached to a 10µl Hamilton syringe. The left eye was used as a control and injected with PBS and BrdU. Animals were sacrificed at different time points (2nd, 7th and 14th day of injection) and eyes were enucleated for fixation using 4% paraformaldehyde. Immunohistochemical analysis was carried out on cryostat eye sections. Results: BrdU positive cells were readily detected in the periphery of the retina and in pigmented ciliary epithelium on 2nd day of intraocular injection. However, the proportion of proliferating cells was higher in growth factor treated eyes than in the controls. BrdU+ cells expressed retinal progenitor markers Chx10 and Pax6. BrdU+ cells were detected in the periphery of retina on 7th day and 14th day of injection and their proportion remained higher in growth factor treated eyes than in the controls. However, the proportion of BrdU+ cells co-expressing proliferation marker Ki-67 decreased significantly on 7th day suggesting that the majority of BrdU positive cells were likely to be postmitotic and did not represent proliferating cells at that stage. On 14th day of injection only 1 or 2 BrdU+ and Ki-67+ cells could be detected within the retinal periphery/section. Conclusions: Our results suggest that exogenous growth factors promote cell proliferation in postnatal retina and ciliary epithelium.

Keywords: retinal development • growth factors/growth factor receptors • cell-cell communication 
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