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S. Kusuhara, M.F. Escano, S. Fujii, A. Negi; Light Deprivation Induces the Increase of Undifferentiated Cells in the Posterior Pole of Post-hatch Chick Eyes . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1685.
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Purpose: Proliferating cells with the potential to differentiate into retinal neurons or glial cells have been reported to exist in the peripheral marginal zone of post-hatch avian retina. We evaluated whether proliferating cells in the posterior pole of post-hatch chick eyes are affected by complete light deprivation. Methods: Right eyes of newly hatched white leghorn chicks were deprived of light stimulation by dark occluders for 3 and 7 days. Right eyes of chicks without occluders were used as controls. After 3 and 7 days of light deprivation, eyes were enucleated and the retinas prepared for sectioning. The posterior poles of sectioned retinas were immunolabeled with the proliferating cell nuclear antigen (PCNA) antibody. Primary cell cultures were done to evaluate for neurosphere formation. Results: There was a significant difference in the average number of retinal cells immunostained with PCNA per section between the control (1.43 ±0.94) and occluded eyes (5.36 ±2.13). Neurosphere formation in the occluded eyes was significantly increased compared with the controls. Conclusion: Proliferating undifferentiated cells in the posterior pole of post-hatch chick retinas increase with light deprivation. In post-hatch chicks, light deprivation may be an important factor in the induction of new neural progenitor cells.
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