May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Ganglion Cells in AMD Retina Express an Epitope of the Glutamate Transporter GLT-1
Author Affiliations & Notes
  • D.V. Pow
    SBMS, Physiology & Pharmacology, University of Queensland, Brisbane, Australia
  • R.K. Sullivan
    SBMS, Physiology & Pharmacology, University of Queensland, Brisbane, Australia
  • E. WoldeMussie
    Allergan Inc, Davis, CA, United States
  • P. Penfold
    Clinical Ophthalmology, Save Sight Inst., University of Sydney, Sydney, Australia
  • Footnotes
    Commercial Relationships  D.V. Pow, None; R.K.P. Sullivan, None; E. WoldeMussie, Allergan E; P. Penfold, RetMed Inc R.
  • Footnotes
    Support  NHMRC (Australia)
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1709. doi:
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    • Get Citation

      D.V. Pow, R.K. Sullivan, E. WoldeMussie, P. Penfold; Ganglion Cells in AMD Retina Express an Epitope of the Glutamate Transporter GLT-1 . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1709.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To examine the expression of a phosphorylation-sensitive epitope of the glutamate transporter GLT-1 in human AMD retinae. Methods: Retinae from post-mortem human donor eyes from Lions Eye Banks in Australia and USA were fixed with 4% paraformaldehyde (1-2 hours). Vibratome sections (40 microns thickness) were immuno-peroxidase-labelled for GLT-1 using our previously characterised antibodies, labelling being revealed using DAB as a chromogen. Results: We demonstrate that whereas previously published studies on human and other mammalian retinae have localised splice variants of GLT-1 to photoreceptors, bipolar cells and some amacrine cells, we now observe, in AMD retinae, that an antibody which usually labels photoreceptors, reveals the presence of strong GLT-1 immunoreactivity in the plasma-membranes of retinal ganglion cells throughout the AMD retina. Conclusions: We conclude that the unusual expression of GLT-1 in retinal ganglion cells in AMD retinae may represent a previously unreported component of the pathology of the disease and may be indicative of underlying glutamate stress in this disease state.

Keywords: ganglion cells • neurotransmitters/neurotransmitter systems • age-related macular degeneration 
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