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M. Tei, M. Kamei, K. Kusaba, T. Yasuhara, C. Mochida, S. Kinoshita; Microarray Gene Expression Analysis of Human Monocytes and Macrophages in Age-Related Macular Degeneration . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1725.
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Purpose: Macrophages have been suggested to play a key role in pathogenesis of subretinal neovascularization. Macrophage gene expression profile in age-related macular degeneration (AMD) was assessed to reveal the pathogenesis of AMD. Methods: Peripheral blood was obtained from two wet type AMD patients. Monocytes and macrophages were purified by the use of a cell sorting system with anti-CD14 antibodies, and total RNA was isolated for cDNA microarray analysis. Peripheral blood from two age and sex matched healthy volunteers was used as the control. cDNA was then labeled using a TSATM kit (PerkinElmer) and hybridized for 16 hours to MicroMaxTM (PerkinElmer) representing a subset of 2400 known human genes. Two Comparisons between normal controls and AMD were conducted. The arrays were scanned with a Laser conforcal scanner (Vertek) and fluorescence intensities were analyzed using MicroArray Suite 2.7.1. Results: Among the 2400 genes, 1060 genes signals were detected in all 4 samples. When compared the normal controls and AMD, 715 genes of the detected 1060 genes showed changes in intensity between 0.5 and 2 fold, 55 genes showed an increase of more than 2 fold and 2 genes decreased. Elongation factor 1 and Wilm’s tumor-related protein were upregulated by more than 3 fold. Conclusions: Although two-thirds of the genes expressed by the monocytes and macrophages were not changed significantly between the controls and AMD, the genes that had significantly changed may be responsible for the key molecules that cause macrophage accumulation in choroidal neovascularization of AMD.
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