May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Microarray Gene Expression Analysis of Human Monocytes and Macrophages in Age-Related Macular Degeneration
Author Affiliations & Notes
  • M. Tei
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-Ku, Kyoto, Japan
  • M. Kamei
    Ophthalmology, Osaka University Medical School, Suita, Osaka, Japan
  • K. Kusaba
    Ophthalmology, Osaka University Medical School, Suita, Osaka, Japan
  • T. Yasuhara
    Ophthalmology, Osaka University Medical School, Suita, Osaka, Japan
  • C. Mochida
    Ophthalmology, Osaka University Medical School, Suita, Osaka, Japan
  • S. Kinoshita
    Ophthalmology, Osaka University Medical School, Suita, Osaka, Japan
  • Footnotes
    Commercial Relationships  M. Tei, None; M. Kamei, None; K. Kusaba, None; T. Yasuhara, None; C. Mochida, None; S. Kinoshita, None.
  • Footnotes
    Support  The Japanease Ministry of Education (14770969)
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1725. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Tei, M. Kamei, K. Kusaba, T. Yasuhara, C. Mochida, S. Kinoshita; Microarray Gene Expression Analysis of Human Monocytes and Macrophages in Age-Related Macular Degeneration . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1725.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Macrophages have been suggested to play a key role in pathogenesis of subretinal neovascularization. Macrophage gene expression profile in age-related macular degeneration (AMD) was assessed to reveal the pathogenesis of AMD. Methods: Peripheral blood was obtained from two wet type AMD patients. Monocytes and macrophages were purified by the use of a cell sorting system with anti-CD14 antibodies, and total RNA was isolated for cDNA microarray analysis. Peripheral blood from two age and sex matched healthy volunteers was used as the control. cDNA was then labeled using a TSATM kit (PerkinElmer) and hybridized for 16 hours to MicroMaxTM (PerkinElmer) representing a subset of 2400 known human genes. Two Comparisons between normal controls and AMD were conducted. The arrays were scanned with a Laser conforcal scanner (Vertek) and fluorescence intensities were analyzed using MicroArray Suite 2.7.1. Results: Among the 2400 genes, 1060 genes signals were detected in all 4 samples. When compared the normal controls and AMD, 715 genes of the detected 1060 genes showed changes in intensity between 0.5 and 2 fold, 55 genes showed an increase of more than 2 fold and 2 genes decreased. Elongation factor 1 and Wilm’s tumor-related protein were upregulated by more than 3 fold. Conclusions: Although two-thirds of the genes expressed by the monocytes and macrophages were not changed significantly between the controls and AMD, the genes that had significantly changed may be responsible for the key molecules that cause macrophage accumulation in choroidal neovascularization of AMD.

Keywords: age-related macular degeneration • pathology: human • neovascularization 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×