May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Resonance Raman Spectroscopic Measurement of Macular Carotenoid Pigments in Patients with Early-Onset Macular Drusen
Author Affiliations & Notes
  • P.S. Bernstein
    Moran Eye Center, University Of Utah, Salt Lake City, UT, United States
  • D. Zhao
    Moran Eye Center, University Of Utah, Salt Lake City, UT, United States
  • S.W. Wintch
    Physics, University Of Utah, Salt Lake City, UT, United States
  • I.V. Ermakov
    Physics, University Of Utah, Salt Lake City, UT, United States
  • R. McClane
    Physics, University Of Utah, Salt Lake City, UT, United States
  • W. Gellermann
    Physics, University Of Utah, Salt Lake City, UT, United States
  • Footnotes
    Commercial Relationships  P.S. Bernstein, Spectrotek P; D. Zhao, None; S.W. Wintch, None; I.V. Ermakov, None; R. McClane, Spectrotek P; W. Gellermann, Spectrotek P.
  • Footnotes
    Support  NIH Grant EY12324; Foundation Fighting Blindness.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 1806. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      P.S. Bernstein, D. Zhao, S.W. Wintch, I.V. Ermakov, R. McClane, W. Gellermann; Resonance Raman Spectroscopic Measurement of Macular Carotenoid Pigments in Patients with Early-Onset Macular Drusen . Invest. Ophthalmol. Vis. Sci. 2003;44(13):1806.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Using ocular resonance Raman spectroscopy, we have previously reported that macular carotenoid levels decline with age and that age-related macular degeneration (AMD) patients have 32% lower levels than age-matched controls (P = 0.001) (Ophthalmology 2002;109:1780-1787). In this study, we examined the macular carotenoid levels and serum lutein levels in patients with early-onset macular drusen to determine if similar abnormalities are present. Methods: 34 normal subjects (14 males, 20 females), age ranging from 19 to 49 (34.4 ± 10.3, M ± SD) and 11 early-onset drusen patients (6 males, 5 females), age ranging from 23 to 49 (38.5 ± 9.5, M ± SD), had macular carotenoid levels measured by resonance Raman spectroscopy, and their blood carotenoid levels were analyzed by HPLC. Results: The mean ± SD of serum lutein levels was 107.8 ± 57.8 and 101 ± 53.3 ng/ml in normal subjects and in early-onset drusen patients, respectively (P = 0.78, Student's t-test). The level of macular carotenoids was 901 ± 407 Raman counts (M ± SD) in normal subjects and 592 ± 397 Raman counts (M ± SD) in early-onset drusen patients (P = 0.049, Student's t-test). Conclusions: Early-onset drusen patients had significantly lower macular pigment levels than age-matched control eyes (34% lower versus normals) in a manner very similar to our previously reported results on AMD eyes. This suggests that low ocular levels of macular carotenoids may play a key role in the macular pathological changes seen in these patients.

Keywords: macular pigment • drusen • nutritional factors 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×