May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Immunohistochemical Localization of Prostaglandin E2 Receptor Subtypes EP1, EP2 and EP4 in Normal Human and Mice Ocular Tissues
Author Affiliations & Notes
  • S. Biswas
    Ophthalmology, University of Louisville, Louisville, KY, United States
  • P. Bhattacherjee
    Ophthalmology, University of Louisville, Louisville, KY, United States
  • C.A. Paterson
    Ophthalmology, University of Louisville, Louisville, KY, United States
  • Footnotes
    Commercial Relationships  S. Biswas, None; P. Bhattacherjee, None; C.A. Paterson, None.
  • Footnotes
    Support  NIH GRANT EY06918
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2080. doi:
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      S. Biswas, P. Bhattacherjee, C.A. Paterson; Immunohistochemical Localization of Prostaglandin E2 Receptor Subtypes EP1, EP2 and EP4 in Normal Human and Mice Ocular Tissues . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2080.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To compare the localization of PGE2 receptor subtypes in normal human and mice ocular tissues. Methods: Paraffin-embedded sections of normal 129 Sv/Ev mice and human eyes were treated with EP1, EP2 and EP4 specific antibodies and subsequently incubated with Alexa Fluor secondary antibody (Ex/Em= 555/571) to detect the presence of EP receptor proteins. Fluorescence of the localized antibodies was visualized in a Nikon Fluorescence microscope and photographed using Kodak TMAX 400 film. Results: In mice, EP1 receptor was only mildly expressed in the corneal and lens epithelia and in retinal ganglion cells. In human eye, EP1 was predominantly expressed in the corneal and ciliary epithelia, longitudinal and circular muscles of ciliary body, retinal ganglion layer and inner plexiform layers and in retinal microvessels. EP2 receptor was the most prominently expressed and widely distributed of all the three subtypes in both human and mice eyes. It was observed almost in all the tissues in the anterior as well as posterior eye. In human eye, EP4 was expressed in corneal endothelium, basal cells of corneal epithelium, trabecular cells, and certain sections in the retina. In mice, it was sparsely expressed in the corneal epithelium, non-pigmented ciliary epithelium, sphincter muscles, lens epithelium, outer plexiform layer and inner segments of photoreceptors. Conclusion: A significant difference is observed in the EP1 and EP4 staining patterns of human and mice eyes. EP1and EP4 receptors expression in the mice ocular tissues seems to be expressed poorly compared with that in the human eye. In contrast, EP2 receptors are strongly expressed in all ocular tissues of both human and mice eyes.

Keywords: inflammation • eicosanoids • receptors: pharmacology/physiology 
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