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M. Nozaki, E. Sakurai, K. Okabe, Y. Ogura; Histopathological Evaluation after Radial Optic Neurotomy in Rabbit . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2218.
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Purpose: Radial optic neurotomy has been recently described as a surgical management for central retinal vein occlusion (CRVO). It is hypothesized that the incision in the cribriform plate and scleral ring will relax scleral outlet and improve blood flow. This study was designed to investigate the histological changes after radial optic neurotomy in the rabbit. Methods:We used pigmented rabbits in this study. The radial incision into the nasal margin of the optic nerve was performed with an MVR blade. At the day of radial optic neurotomy, 1 week and 1 month after neurotomy, the eyes were enucleated. The enucleated eyes were fixed in 4% paraformaldehyde and 0.1 M phosphate buffer. Hematoxylin and eosin staining was performed. The optic nerves were stained immunohistochemically for expression of GFAPglial fibrillary acid protein), vimentin to visualize the astrocytes and CNPase2’,3’-cyclic nucleotide phosphohydrolase) to visualize the oligodendrocytes by sABC method. Results:Macroscopically, at the day of radial optic neurotomy, vitreous hemorrhage was detected, but at 1 week and 1 month after neurotomy, vitreous hemorrhage was not detected. Microscopically, the globe was not ruptured in any eye. The central retinal vein and artery remained undisturbed. Immunohistochemically, until 1 month after neurotomy, GFAP and vimentin were increased. There was no difference in immunostaining with CNPase between control and operated optic nerves. Conclusions: These results demonstrate that radial optic neurotomy in the rabbit does not damage to the central retinal vein and artery. The enhanced expression of GFAP and vimentin indicated the activation of astrocytes.
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