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G. Zhan, C.B. Toris, S.J. Mann, J. Meza, A.M. LeDay, C.B. Camras; Effect of a Cyclooxygenase (COX) Inhibitor on Iris Color Darkening Induced by Topical Latanoprost in Sympathectomized (SX) Rabbit Eyes . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2240.
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Purpose: To determine whether diclofenac, a COX inhibitor, can inhibit iris color darkening in SX rabbit eyes treated with latanoprost, a prostaglandin F2α analogue. Methods: Thirteen Dutch belted rabbits underwent bilateral superior cervical ganglionectomy. Starting the next day and for seven months, diclofenac 0.1% was administered four times a day to one eye and vehicle to the contralateral eye in a randomized, masked fashion, and both eyes were treated with latanoprost 0.005% twice-daily. Color photographs of rabbit irides were taken at monthly intervals. Observers rated paired photographs using scores ranging from "0" for no obvious decision as to a difference in iris color between paired eyes, to "3" for very certain of a color difference. The scores were analyzed using a random effects model. Scheffe multiple comparisons were made to compare scores by months. At seven months, aqueous humor was measured for protein and PGE2 levels, and iris tissue was assayed for tyrosinase. Values from diclofenac-treated vs vehicle treated eyes were compared by a paired t-test. Results: Based on an analysis of observer scores, the latanoprost/diclofenac-treated eyes were judged to be lighter than the contralateral latanoprost/vehicle- treated eyes starting at month 1 and continuing through month 7 (p=0.0001). There were no differences between eyes in protein or PGE2 levels in the aqueous humor, or tyrosinase in the iris. Conclusions: Diclofenac can inhibit the iris color darkening induced by treatment with latanoprost in an experimental rabbit model.
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