May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Expression of Novel Secretory Phospholipases A2 in Rat Retina
Author Affiliations & Notes
  • M. Kolko
    Ophthalmology and Neuroscience, LSU Health Sciences Center, New Orleans, LA, United States
  • N.R. Christoffersen
    Ophthalmology and Neuroscience, LSU Health Sciences Center, New Orleans, LA, United States
  • H. Varoqui
    Ophthalmology and Neuroscience, LSU Health Sciences Center, New Orleans, LA, United States
  • N.G. Bazan
    Ophthalmology and Neuroscience, LSU Health Sciences Center, New Orleans, LA, United States
  • Footnotes
    Commercial Relationships  M. Kolko, None; N.R. Christoffersen, None; H. Varoqui, None; N.G. Bazan, None.
  • Footnotes
    Support  NEI R01EY05121
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2266. doi:
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      M. Kolko, N.R. Christoffersen, H. Varoqui, N.G. Bazan; Expression of Novel Secretory Phospholipases A2 in Rat Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2266.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Low-molecular-weight phospholipases A2s (sPLA2s) are a subgroup of PLA2s, which are secreted and bind to receptors. At least ten different groups have been characterized in mammals and there is expanding evidence on the importance of sPLA2s in neuronal signaling and survival [Kolko et al. (1996) J Biol Chem 271: 32722-32728]. Novel sPLA2 activity has been meassured in the retina [e.g. Giusto et al., (2000) Prog Lipid Res 39: 315-391], but to date none has been cloned and characterized. Here we evaluated the existence and abundance of novel sPLA2s in rat retina by PCR cloning and real-time PCR and explored their possible involvement in light damage. Methods: Based on a known sequence of sPLA2 group IB, X, V, IIE, IIA, IIF, and IID in rat pancreas, rat lung, rat brain, mouse brain, rat platelets, and mouse spleen, respectively, we designed primers to identify the sPLA2s in rat retina. RNA was isolated from adult rat retina and cDNA was produced and used for PCR cloning to identify the novel subtypes of sPLA2s. We confirmed the products by sequencing. Primers were designed for real-time PCR based on our cloned sequence. Light damage was induced by bright fluorescent light for 5 hr followed by darkness until rats were killed after 0, 2, 4, 6, 12, and 24 hr and retinas were isolated [Gordon et al., (2002) IOVS, 43: 3511-3521]. Results: Using PCR cloning we identified sPLA2-IB, X, V, IIE, IIA, IIF, and IID in the normal rat retina. By real-time PCR we quantified four subgroups of sPLA2s and found sPLA2-IB to be the most abundant sPLA2 followed by group V, IIE, and X, respectively. Finally, we showed a time-dependent induction of sPLA2 in rats exposed to light damage. sPLA2-IB and X were induced 4 and 12 hr after light damage by approximately 5 and 12 fold, respectively, whereas sPLA2-V and IIE were only induced at most 3.5 fold, 4 hr after light damage. Conclusions: This study is the first to reveal the existence and abundance of specific sPLA2s in rat retina. Furthermore, we show that the subtypes are differentially induced during light damage, suggesting different roles of the sPLA2s in retina. These enzymes may be intercellular signaling modulators significant in retina cell function and in retinal degenerations.

Keywords: retina • gene/expression • molecular biology 
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