Purchase this article with an account.
G. Garcia-Aguirre, J.L. Guerrero-Naranjo, L.M. Suarez-Tata, S. Murillo-Lopez, G. Reategui-Escalante, C. Leizaola-Fernandez, L. Morfin-Salido, D. Rios-Valles, A. Rodriguez-Reyes, H. Quiroz-Mercado; Toxicity Study of Perfluorocarbon Liquids vs. Balanced Saline Solution in Human Lenses . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2353.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To compare the degree of opacification of human lenses immersed in perfluorocarbon liquids (PFCLs) vs. balanced saline solution (BSS). Methods: Prospective, longitudinal, experimental and descriptive study. We used ten crystalline lenses from ten babies (age range, 4 -24 months) with stage 5 retinopathy of prematurity that underwent an open-sky vitrectomy. Use of human tissue in this study conformed to the tenets of the Declaration of Helsinki and parents signed a written consent approved by the Institutional Review Board. In all eyes, intraocular open sky cryoextraction (exactly six seconds) of a clear crystalline lens was performed by the same surgeon using the same surgical technique. Immediately after extraction, lenses were placed in separated glass vials; six were immersed in perfluoro-n-octane and four in BSS; all were incubated for 10 days at 37°C. Evaluation of the transparency was done by daily digital photography. At the end of the observation period, lens opacification was arbitrarily graded from 0 to 3 (grade 0, no opacification; grade 1, minimal; grade 2: moderate; grade 3: total opacification). Results: At the end of the observation period (day 10), lenses immersed in BSS were completely opacified (grade 3) and the lenses immersed in PFCLs were partly opacified (grades 1 and 2). Histologic study revealed no difference between each group. Conclusions: Human baby lenses incubated in PFCLs remained relatively clear as opposed to lens in BSS. An isolation effect with decrease in oxidation and hydration should be considered.
This PDF is available to Subscribers Only