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V. Jain, E.K. Wong Jr., H. Agnic, S. Vong, E. Kang, C. Lee, A. Nafissi, J. Soriano, Y. Pham, R. Kinyamu; A Rat Model of Neurotrophic Keratitis: Anatomical and Physiological Studies . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2445.
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Purpose: Our research is focused on creating a reproducible model of Neurotrophic Keratitis (NK), a result of sensory denervation of the cornea via the ophthalmic branch of the trigeminal nerve (V1). The literature shows that an animal model is needed to study the efficacy of therapies and pathophysiology of this disease. Methods: To denervate the cornea we create a lesion in V1 using neurosurgical radiofrequency thermocautery (Radionics, #RFG-3C) administered through a stereotactic instrument with the animal lying supine. Recently, we have begun stimulating the nerve with up to 0.20 volts before creating the lesion. To further clarify the anatomy of the branching of the trigeminal nerve (CN V), we performed 25 microdissections of Sprague-Dawley rats between 190-430g. View OriginalDownload SlideView OriginalDownload Slide
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