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A.P. Dálmeida, L.M. Cardoso; Effect of Estrogen-based Eye Drops on Female Rabbits . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2496.
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Purpose: To look at the toxic effect of using estrogen-based eye drops at different concentrations on the corneoconjunctival epithelium in female rabbits. Methods: 15 3-month old female albino rabbits weighing approximately 4 to5 kg were used.They were divided into 5 groups. Eye drops were instilled containing estrogen conjugated at different diluitions for each group and then the avaliation of the corneoconjunctival epithelium was measured using two methods: 1)Bengal Rose -The animals were examined under a slit lamp with a cobalt blue filter after the 1% Bengal Rose eye drops were instillated. The color was classified on the following scale: -+discrete positivity -++ moderate positvity -+++ great posivity 2)Conjuntival cytology-Anesthesic eye drops were instilated into the cul de sac of the conjunctiva,in both eyes and later ,using a Kimura spatula ,the lower palpebral conjunctiva was scraped and fixed on a slide.Then it was stained using the Giemsa method,and interpreted according to the following classification: I-normal epithelial cells II-moderately Keratinized epithelial cells III-Keratinized epithelial cells Four samplings were performed after instillating the estrogen-based eye drops in five different dilutions. The first group received eye drops with 100% estrogens.The second received eye drops with 50% estrogens, the third with 25% ,the fourth with 12,50% and the fifth with 6.2%.Dilution was performed with methylcellulose at 0.5%. The eye drops were instillated in the rabbits four times a day,every 6 hours.The first sampling was performed one week later,the second after fifteen days,and the third after twenty-one days Results: After one week: GROUP 1-BENGAL ROSE-+ CITOLOGY-I GROUP 2-BENGAL ROSE-++ CITOLOGY-II GROUP 3-BENGAL ROSE-++ CITOLOGY-II GROUP 4-BENGAL ROSE-+ CITOLOGY-I GROUP 5-BENGAL ROSE-+ CITOLOGY-I After 2 weeks: GROUP 1-BENGAL ROSE-+ + CITOLOGY-I GROUP 2-BENGAL ROSE-+ CITOLOGY-I GROUP 3-BENGAL ROSE -++ CITOLOGY-II GROUP 4-BENGAL ROSE-+ CITOLOGY-I GROUP 5-BENGAL ROSE-+ CITOLOGY-I After 3 weeks: GROUP 1-BEBGAL ROSE-+ CITOLOGY-I GROUP 2-BENGAL ROSE-++ CITOLOGY-II GROUP 3 BENGAL ROSE-++ CITOLOGY-II GROUP 4-BENGAL ROSE-+ CITOLOGY-I GROUP 5-BENGAL ROSE-+ CITOLOGY-I Conclusions: In this study showed us that the estrogen-based eye drops at different concentrations did not present any toxic effect,as shown by the citology and by the Bengal Rose coloring on the corneoconjunctival epithelium in female rabbits.
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